Publications

Cell Death & Differentiation Jun 18. doi: 10.1038/s41418-022-01030-y. Online ahead of print. PMID: 35717504

Designed Ankyrin Repeat Proteins as a tool box for analyzing p63

Strubel A, Münick P, Chaikuad A, Dreier B, Schaefer J, Gebel J, Osterburg C, Tuppi M, Schäfer B, Knapp S, Plückthun A, Dötsch V

Details

Human Mutation Jul 8. doi: 10.1002/humu.24432. Online ahead of print. PMID: 35801529

Dominant TP63 missense variants lead to constitutive activation and premature ovarian insufficiency

Tucker EJ, Gutfreund N, Belaud-Rotureau MA, Gilot D, Brun T, Kline BL, Domin-Bernhard M, Théard C, Touraine P, Robevska G, van den Bergen J, Ayers KL, Sinclair AH, Dötsch V, Jaillard S.

Details

Cell Death Dis Mar 4;13(3):204. doi: 10.1038/s41419-022-04659-2. PMID: 35246516 Free PMC article

Enhanced pro-apoptosis gene signature following the activation of TAp63α in oocytes upon γ irradiation

Fester N, Zielonka E, Goldmann J, Frombach AS, Müller-Kuller U, Gutfreund N, Riegel K, Smits JGA, Schleiff E, Rajalingam K, Zhou H, Simm S, Dötsch V.

Details

J Mol Biol. Jun 16;434(16):167687. doi: 10.1016/j.jmb.2022.167687. Online ahead of print. PMID: 35717996

Biochemical Characterization of Cell-free Synthesized Human β1 Adrenergic Receptor Cotranslationally Inserted into Nanodiscs

Köck Z, Ermel U, Martin J, Morgner N, Frangakis AS, Dötsch V, Hilger D, Bernhard F

Details

Mol Cell. Jun 2;82(11):2006-2020.e8. doi: 10.1016/j.molcel.2022.03.005. Epub 2022 Mar 29. PMID: 35353987 Free PMC article

Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes

Cullati SN, Chaikuad A, Chen JS, Gebel J, Tesmer L, Zhubi R, Navarrete-Perea J, Guillen RX, Gygi SP, Hummer G, Dötsch V, Knapp S, Gould KL

Details

Oncogene Jan;41(3):372-386. doi: 10.1038/s41388-021-02094-9. Epub 2021 Nov 10. PMID: 34759346 Free PMC article.

A dimerization-dependent mechanism regulates enzymatic activation and nuclear entry of PLK1

Raab M, Matthess Y, Raab CA, Gutfreund N, Dötsch V, Becker S, Sanhaji M, Strebhardt K

Details

Methods Mol Biol. 2507:405-424. doi: 10.1007/978-1-0716-2368-8_22. PMID: 35773595

Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies

Umbach S, Dötsch V, Bernhard F.

Details

Methods Mol Biol. 2406:245-266. doi: 10.1007/978-1-0716-1859-2_15. PMID: 350895

Applications of Cell-Free Synthesized Membrane Protein Precipitates

Mezhyrova J, Mörs K, Glaubitz C, Dötsch V, Bernhard F.

Details

Cell Death Differ. May;29(5):921-937. doi: 10.1038/s41418-022-00975-4. Epub 2022 Mar 21. PMID: 35314772 Free PMC article. Review

Structural diversity of p63 and p73 isoforms

Osterburg C, Dötsch V.

Details

J Med Chem. Aug 12;64(15):10682-10710. doi: 10.1021/acs.jmedchem.1c00146. Epub 2021 May 13. PMID: 33980013

Design, Synthesis, and Evaluation of WD-Repeat-Containing Protein 5 (WDR5) Degraders

Dölle A, Adhikari B, Krämer A, Weckesser J, Berner N, Berger LM, Diebold M, Szewczyk MM, Barsyte-Lovejoy D, Arrowsmith CH, Gebel J, Löhr F, Dötsch V, Eilers M, Heinzlmeir S, Kuster B, Sotriffer C, Wolf E, Knapp S.

Details

EMBO J. Apr 19;41(8):e108443. doi: 10.15252/embj.2021108443. Epub 2022 Feb 3. PMID: 35112738 Free PMC article.

Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation

Gruijs da Silva LA, Simonetti F, Hutten S, Riemenschneider H, Sternburg EL, Pietrek LM, Gebel J, Dötsch V, Edbauer D, Hummer G, Stelzl LS, Dormann D.

Details

J Med Chem. 2021 Mar 26. doi: 10.1021/acs.jmedchem.0c01564. Online ahead of print. PMID: 33769048

Demonstrating Ligandability of the LC3A and LC3B Adapter Interface.

Hartmann M, Huber J, Kramer JS, Heering J, Pietsch L, Stark H, Odadzic D, Bischoff I, Fürst R, Schröder M, Akutsu M, Chaikuad A, Dötsch V, Knapp S, Biondi RM, Rogov VV, Proschak E.

Details

Cell Death Differ. doi: 10.1038/s41418-021-00766-3. Online ahead of print. PMID: 33723372

Characterization of a natural variant of human NDP52 and its functional consequences on mitophagy

Di Rita A, Angelini DF, Maiorino T, Caputo V, Cascella R, Kumar M, Tiberti M, Lambrughi M, Wesch N, Löhr F, Dötsch V, Carinci M, D'Acunzo P, Chiurchiù V, Papaleo E, Rogov VV, Giardina E, Battistini L, Strappazzon F.

Details

EMBO J. e106094. doi: 10.15252/embj.2020106094. Online ahead of print. PMID: 33576509

The UBA domain of conjugating enzyme Ubc1/Ube2K facilitates assembly of K48/K63-branched ubiq

Pluska L, Jarosch E, Zauber H, Kniss A, Waltho A, Bagola K, von Delbrück M, Löhr F, Schulman BA, Selb

Details

Cancers (Basel) 13(3):536. doi: 10.3390/cancers13030536. PMID: 33572532 Free PMC article. Review.

Isoform-Specific Roles of Mutant p63 in Human Diseases

Osterburg C, Osterburg S, Zhou H, Missero C, Dötsch V.

Details

Nat Commun. 12(1):383. doi: 10.1038/s41467-020-20669-0. PMID: 33452256

The p63 C-terminus is essential for murine oocyte integrity

Lena AM, Rossi V, Osterburg S, Smirnov A, Osterburg C, Tuppi M, Cappello A, Amelio I, Dötsch V, De Felici M, Klinger FG, Annicchiarico-Petruzzelli M, Valensise H, Melino G, Candi E.

Details

Cell Chem Biol S2451-9456(20)30283-X. doi: 10.1016/j.chembiol.2020.07.010. Online ahead of print. PMID: 32726587

Discovery of Protein-Protein Interaction Inhibitors by Integrating Protein Engineering and Chemical S

Maculins T, Garcia-Pardo J, Skenderovic A, Gebel J, Putyrski M, Vorobyov A, Busse P, Varga G, Kuzikov M, Zaliani A, Rahighi S, Schaeffer V, Parnham MJ, Sidhu SS, Ernst A, Dötsch V, Akutsu M, Dikic I.

Details

J Vis Exp (165). doi: 10.3791/61844

Co-translational Insertion of Membrane Proteins into Preformed Nanodiscs

Levin R, Koeck Z, Dötsch V, Bernhard F.

Details

FEBS J. doi: 10.1111/febs.15642. Online ahead of print. PMID: 33244868

Membrane insertion mechanism and molecular assembly of the bacteriophage lysis toxin ΦX174-E

Mezhyrova J, Martin J, Peetz O, Dötsch V, Morgner N, Ma Y, Bernhard F.

Details

Int J Mol Sci. 21(15):E5369. doi: 10.3390/ijms21155369. PMID: 32731622 Review.

Ubiquitination in the ERAD Process

Lopata A, Kniss A, Löhr F, Rogov VV, Dötsch V.

Details

Cell Chem Biol S2451-9456(20)30283-X. doi: 10.1016/j.chembiol.2020.07.010. Online ahead of print. PMID: 32726587

Discovery of Protein-Protein Interaction Inhibitors by Integrating Protein Engineering and Chemical S

Maculins T, Garcia-Pardo J, Skenderovic A, Gebel J, Putyrski M, Vorobyov A, Busse P, Varga G, Kuzikov M, Zaliani A, Rahighi S, Schaeffer V, Parnham MJ, Sidhu SS, Ernst A, Dötsch V, Akutsu M, Dikic I.

Details

Nat Chem Biol doi: 10.1038/s41589-020-0600-3. Online ahead of print. PMID: 32719556

p63 uses a switch-like mechanism to set the threshold for induction of apoptosis.

Gebel J, Tuppi M, Chaikuad A, Hötte K, Schröder M, Schulz L, Löhr F, Gutfreund N, Finke F, Henrich E, Mezhyrova J, Lehnert R, Pampaloni F, Hummer G, Stelzer EHK, Knapp S, Dötsch V.

Details

J Biol Chem pii: jbc.RA119.010315. doi: 10.1074/jbc.RA119.010315. [Epub ahead of print] PMID: 31937588 [PubMed - as supplied by publisher]

Oxygen-dependent asparagine hydroxylation of the ubiquitin-associated (UBA) domain in Cezanne re

Mader J, Huber J, Bonn F, Doetsch V, Rogov VV, Bremm A.

Details

Mol Cell pii: S1097-2765(19)30797-X. doi: 10.1016/j.molcel.2019.10.019. [Epub ahead of print] PMID: 31732457 [PubMed - as supplied by publisher] Free Article

Regulation of Phosphoribosyl-Linked Serine Ubiquitination by Deubiquitinases DupA and DupB

Shin D, Mukherjee R, Liu Y, Gonzalez A, Bonn F, Liu Y, Rogov VV, Heinz M, Stolz A, Hummer G, Dötsch V, Luo ZQ, Bhogaraju S, Dikic I.

Details

J Invest Dermatol pii: S0022-202X(19)33376-7. doi: 10.1016/j.jid.2019.06.154. [Epub ahead of print] No abstract available. PMID: 31682841 [PubMed - as supplied by publisher]

A TP63 mutation causes prominent alopecia with mild ectodermal dysplasia

A. Duchatelet S, Russo C, Osterburg C, Mallet S, Bole-Feysot C, Nitschké P, Richard MA, Dötsch V, Missero C, Nassif A, Hovnanian A.

Details

Mol Cell pii: S1097-2765(19)30690-2. doi: 10.1016/j.molcel.2019.09.005. [Epub ahead of print] Review. PMID: 31585693 [PubMed - as supplied by publisher]

A Diversity of Selective Autophagy Receptors Determines the Specificity of the Autophagy Pathway

Kirkin V, Rogov VV.

Details

Cell Death Dis 10(10):686. doi: 10.1038/s41419-019-1936-z. PMID: 31527723 [PubMed - in process]

TA*p63 and GTAp63 achieve tighter transcriptional regulation in quality control by converting an inhibitory element into an additional transactivation domain

Pitzius S, Osterburg C, Gebel J, Tascher G, Schäfer B, Zhou H, Münch C, Dötsch V.

Details

Nat Commun 10(1):4176. doi: 10.1038/s41467-019-12094-9. PMID: 31519908 [PubMed - in process] Free PMC Article

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites

Holdgaard SG, Cianfanelli V, Pupo E, Lambrughi M, Lubas M, Nielsen JC, Eibes S, Maiani E, Harder LM, Wesch N, Foged MM, Maeda K, Nazio F, de la Ballina LR, Dötsch V, Brech A, Frankel LB, Jäättelä M, Locatelli F, Barisic M, Andersen JS, Bekker-Jensen S, Lund AH, Rogov VV, Papaleo E, Lanzetti L, De Zio D, Cecconi F

Details

Autophagy doi: 10.1080/15548627.2019.1606637. [Epub ahead of print] PMID: 30990354 [PubMed - as supplied by publisher]

An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5

Huber J, Obata M, Gruber J, Akutsu M, Löhr F, Rogova N, Güntert P, Dikic I, Kirkin V, Komatsu M, Dötsch V, Rogov VV

Details

J Magn Reson 302:50-63. doi: 10.1016/j.jmr.2019.03.010. [Epub ahead of print] PMID: 30959416 [PubMed - as supplied by publisher]

Towards complete polypeptide backbone NH assignment via combinatorial labeling

Löhr F, Gebel J, Henrich E, Hein C, Dötsch V.

Details

Eur J Hum Genet doi: 10.1038/s41431-019-0370-0. [Epub ahead of print] PMID: 30850703 [PubMed - as supplied by publisher]

Deletions and loss-of-function variants in TP63 associated with orofacial clefting

Khandelwal KD, van den Boogaard MH, Mehrem SL, Gebel J, Fagerberg C, van Beusekom E, van Binsbergen E, Topaloglu O, Steehouwer M, Gilissen C, Ishorst N, van Rooij IALM, Roeleveld N, Christensen K, Schoenaers J, Bergé S, Murray JC, Hens G, Devriendt K, Ludwig KU, Mangold E, Hoischen A, Zhou H, Dötsch V, Carels CEL, van Bokhoven H.

Details

Cell Death Differ doi: 10.1038/s41418-019-0298-5. [Epub ahead of print] PMID: 30742091 [PubMed - as supplied by publisher]

Cell cycle arrest in mitosis promotes interferon-induced necroptosis

Frank T, Tuppi M, Hugle M, Dötsch V, van Wijk SJL, Fulda S.

Details

Methods Enzymol 614:143-185. doi: 10.1016/bs.mie.2018.08.019. Epub 2018 Dec 21. PMID: 30611423

Synthetic Biology-Based Solution NMR Studies on Membrane Proteins in Lipid Environments

Henrich E, Löhr F, Mezhyrova J, Laguerre A, Bernhard F, Dötsch V.

Details

EMBO Rep. 2018 Dec;19(12). pii: e47268. doi: 10.15252/embr.201847268. No abstract available.

Structural and functional analysis of the GABARAP interaction motif (GIM)

Rogov VV, Stolz A, Ravichandran AC, Rios-Szwed DO, Suzuki H, Kniss A, Löhr F, Wakatsuki S, Dötsch V, Dikic I, Dobson RC, McEwan DG.

Details

PLoS One. 2017 Dec 14;12(12):e0189532. doi: 10.1371/journal.pone.0189532. eCollection 2017. PMID: 29240839

The soluble loop BC region guides, but not dictates, the assembly of the transmembrane cytochrome b6

Tome-Stangl L, Schaetzel C, Tenzer S, Bernhard F, Schneider D.

Details

Mol Pharmacol. 2018 Nov 8. pii: mol.118.113498. doi: 10.1124/mol.118.113498. [Epub ahead of print] PMID: 30409791

Rat Organic Cation Transporter 1 Contains Three Binding Sites for Substrate 1-Methyl-4-phenylpyridinium per Monomer

Keller T, Gorboulev V, Mueller T, Dotsch V, Bernhard F, Koepsell H.

Details

Sci Rep. 2018 Oct 23;8(1):15662. doi: 10.1038/s41598-018-33841-w.

Structural and functional insights into the interaction and targeting hub TMD0 of the polypeptide transporter TAPL.

Bock C, Löhr F, Tumulka F, Reichel K, Würz J, Hummer G, Schäfer L, Tampé R, Joseph B, Bernhard F, Dötsch V, Abele R.

Details

J Mol Biol. 2018 Oct 18. pii: S0022-2836(18)30556-4. doi: 10.1016/j.jmb.2018.10.006. [Epub ahead of print]

Molecular Determinants for Ligand Selectivity of the Cell-Free Synthesized Human Endothelin B Receptor.

Dong F, Rues RB, Kazemi S, Dötsch V, Bernhard F.

Details

Biochemistry. 2018 Sep 18. doi: 10.1021/acs.biochem.8b00807. [Epub ahead of print] PMID: 30226041

Lipid conversion by cell-free synthesized phospholipid methyltransferase Opi3 in defined nanodisc membranes supports in trans mechanism

Henrich E, Lőhr F, Pawlik G, Peetz O, Dötsch V, Morgner N, Kroon AI, Bernhard F.

Details

J Am Soc Mass Spectrom. 2018 Sep 17. doi: 10.1007/s13361-018-2061-4. [Epub ahead of print] PMID: 30225732

LILBID and nESI: Different Native Mass Spectrometry Techniques as Tools in Structural Biology.

Peetz O, Hellwig N, Henrich E, Mezhyrova J, Dötsch V, Bernhard F, Morgner N.

Details

Nat Commun. 2018 Sep 14;9(1):3755. doi: 10.1038/s41467-018-05722-3. PMID: 30217973

HUWE1 E3 ligase promotes PINK1/PARKIN-independent mitophagy by regulating AMBRA1 activation via IKKα

Di Rita A, Peschiaroli A, D Acunzo P, Strobbe D, Hu Z, Gruber J, Nygaard M, Lambrughi M, Melino G, Papaleo E, Dengjel J, El Alaoui S, Campanella M, Dötsch V, Rogov VV, Strappazzon F, Cecconi F.

Details

Structure. 2018 Aug 7;26(8):1091-1100.e4. doi: 10.1016/j.str.2018.05.013. Epub 2018 Jun 28. PMID: 30099987

Regulation of the Activity in the p53 Family Depends on the Organization of the Transactivation Domain.

Krauskopf K, Gebel J, Kazemi S, Tuppi M, Löhr F, Schäfer B, Koch J, Güntert P, Dötsch V, Kehrloesser S.

Details

J Biomol NMR. 2018 Jul 31. doi: 10.1007/s10858-018-0201-6. [Epub ahead of print] PMID: 30066206

Structural investigation of glycan recognition by the ERAD quality control lectin Yos9

Kniss A, Kazemi S, Löhr F, Berger M, Rogov VV, Güntert P, Sommer T, Jarosch E, Dötsch V.

Details

ACS Chem Biol. 2018 Jun 12. doi: 10.1021/acschembio.8b00338. [Epub ahead of print] PMID: 29894149

Precursor-based selective methyl labelling of cell-free synthesized proteins

Lazarova M, Lőhr F, Rues RB, Kleebach R, Dötsch V, Bernhard F.

Details

Elife. 2018 Apr 20;7. pii: e34311. doi: 10.7554/eLife.34311. PMID: 29676732

Donated chemical probes for open science

Müller S, Ackloo S, Arrowsmith CH, Bauser M, Baryza JL, Blagg J, Böttcher J, Bountra C, Brown PJ, Bunnage ME, Carter AJ, Damerell D, Dötsch V, Drewry DH, Edwards AM, Edwards J, Elkins JM, Fischer C, Frye SV, Gollner A, Grimshaw CE, IJzerman A, Hanke T, Hartung IV, Hitchcock S, Howe T, Hughes TV, Laufer S, Li VM, Liras S, Marsden BD, Matsui H, Mathias J, O'Hagan RC, Owen DR, Pande V, Rauh D, Rosenberg SH, Roth BL, Schneider NS, Scholten C, Singh Saikatendu K, Simeonov A, Takizawa M, Tse C, Thompson PR, Treiber DK, Viana AY, Wells CI, Willson TM, Zuercher WJ, Knapp S, Mueller-Fahrnow A.

Details

Cell Death Differ. 2018 Apr 17. doi: 10.1038/s41418-018-0107-6. [Epub ahead of print] No abstract available. PMID: 29666471

CHK2 sets the stage for CK1 in oocyte quality control

Kehrloesser S, Tuppi M, Dötsch V.

Details

Chemistry. 2018 Apr 14. doi: 10.1002/chem.201801770. [Epub ahead of print] PMID: 29656465

Structural Characterization of the Interaction of the Fibroblast Growth Factor Receptor with a Small Molecule Allosteric Inhibitor

Kappert F, Sreeramulu S, Jonker HRA, Richter C, Rogov VV, Proschak E, Hargittay B, Saxena K, Schwalbe H.

Details

Prog Nucl Magn Reson Spectrosc. 2018 Apr;105:1-22. doi: 10.1016/j.pnmrs.2017.11.004. Epub 2017 Dec 7. Review. PMID: 29548364

Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis

Hoffmann B, Löhr F, Laguerre A, Bernhard F, Dötsch V.

Details

Nat Struct Mol Biol. 2018 Feb 26. doi: 10.1038/s41594-018-0035-7. [Epub ahead of print] PMID: 29483652

Oocyte DNA damage quality control requires consecutive interplay of CHK2 and CK1 to activate p63

Tuppi M, Kehrloesser S, Coutandin DW, Rossi V, Luh LM, Strubel A, Hötte K, Hoffmeister M, Schäfer B, De Oliveira T, Greten F, Stelzer EHK, Knapp S, De Felici M, Behrends C, Klinger FG, Dötsch V.

Details

Methods. 2018 Jan 26. pii: S1046-2023(17)30386-9. doi: 10.1016/j.ymeth.2018.01.012. [Epub ahead of print] PMID:29378315

Systematic optimization of cell-free synthesized human endothelin B receptor folding.

Rues RB, Dong F, Dötsch V, Bernhard F

Details

Structure. 018 Jan 17. pii: S0969-2126(17)30410-0. doi: 10.1016/j.str.2017.12.011. [Epub ahead of print] PMID:29358025

Chain Assembly and Disassembly Processes Differently Affect the Conformational Space of Ubiquitin Chains.

Kniss A, Schuetz D, Kazemi S, Pluska L, Spindler PE, Rogov VV, Husnjak K, Dikic I, Güntert P, Sommer T, Prisner TF, Dötsch V.

Details

Proc Natl Acad Sci U S A. Proc Natl Acad Sci U S A.

Protein aggregation of the p63 transcription factor underlies severe skin fragility in AEC syndrome.

Russo C, Osterburg C, Sirico A, Antonini D, Ambrosio R, Würz JM, Rinnenthal J, Ferniani M, Kehrloesser S, Schäfer B, Güntert P, Sinha S, Dötsch V, Missero C.

Details

Nat Commun. 2017 Dec 13;8(1):2101. doi: 10.1038/s41467-017-02313-6. PMID:29235475

Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.

Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O.

Details

Anal Chem. Non-oncogenic roles of TAp73: from multiciliogenesis to metabolism.

Non-oncogenic roles of TAp73: from multiciliogenesis to metabolism.

Non-oncogenic roles of TAp73: from multiciliogenesis to metabolism.

Details

J Biol Chem. 2017 Aug 7. pii: jbc.M117.794255. doi: 10.1074/jbc.M117.794255. [Epub ahead of print] PMID: 28784659

The E. coli S30 lysate proteome: A prototype for cell-free protein production.

Wiechmann S, Gärtner A, Kniss A, Stengl A, Behrends C, Rogov VV, Rodriguez MS, Dötsch V, Müller S, Ernst A.

Details

PlosOne. (2017)

The soluble loop BC guides, but not dictates, the assembly of the transmembrane cytochrome b6.

Tome, L., Schätzel, C., Tenzer, S., Bernhard, F., and Schneider,

Details

Anal. Chem. 89 12314-12318

Insights into co-translational membrane protein insertion by combined LILBID-mass spectrometry and NMR spectroscopy.

Peetz, O., Henrich, E., Laguerre, A., Löhr, F., Hein, C., Dötsch, V., Bernhard, F., and Morgner, N.

Details

N Biotechnol. 2017 Jul 6. pii: S1871-6784(16)32503-1. doi: 10.1016/j.nbt.2017.06.014. [Epub ahead of print] Review. PMID: 28690157

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

Jia H, Heymann M, Bernhard F, Schwille P, Kai L.

Details

EMBO Rep. 2017 Jun 27. pii: e201643587. doi: 10.15252/embr.201643587. [Epub ahead of print] PMID: 28655748

Structural and functional analysis of the GABARAP interaction motif (GIM)

Rogov VV, Stolz A, Ravicahandran AC, Rios-Szwed DO, Suzuki H, Kniss A, Löhr F, Wakatsuki S, Dötsch V, Dikic I, Dobson RC, McEwan DG.

Details

Methods Mol Biol. 2017;1586:291-312. doi: 10.1007/978-1-4939-6887-9_19. PMID: 28470613

Membrane Protein Production in E. coli Lysates in Presence of Preassembled Nanodiscs.

Rues RB, Gräwe A, Henrich E, Bernhard F.

Details

Biophys J. 2017 Apr 24. pii: S0006-3495(17)30345-4. doi: 10.1016/j.bpj.2017.03.026. PMID: 28450130

From Gene to Function: Cell-free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs.

Henrich E, Sörmann J, Eberhardt P, Peetz O, Mezhyrova J, Morgner N, Fendler K, Dötsch V, Wachtveitl J, Bernhard F, Bamann C.

Details

Sci Rep. 2017 Apr 25;7(1):1131. doi: 10.1038/s41598-017-01258-6. PMID: 28442745

Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins.

Rogov VV, Suzuki H, Marinković M, Lang V, Kato R, Kawasaki M, Buljubašić M, Šprung M, Rogova N, Wakatsuki S, Hamacher-Brady A, Dötsch V, Dikic I, Brady NR, Novak I.

Details

EMBO Rep. 2017 Mar 23. pii: e201643744. doi: 10.15252/embr.201643744. [Epub ahead of print] PMID: 28336776

Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2.

Imre G, Berthelet J, Heering J, Kehrloesser S, Melzer IM, Lee BI, Thiede B, Dötsch V, Rajalingam K.

Details

Methods Enzymol. 2017;587:143-169. doi: 10.1016/bs.mie.2016.10.023. PMID: 28253953

Methods for Studying Interactions Between Atg8/LC3/GABARAP and LIR-Containing Proteins.

Johansen T, Birgisdottir ÅB, Huber J, Kniss A, Dötsch V, Kirkin V, Rogov VV.

Details

Growth Des. 17, 945-948.

Integral Membrane Proteins Can Be Crystallized Directly from Nanodiscs.

M. Nikolaev, E. Round, I. Gushchin, V. Polovinkin, T. Balandin, P. Kuzmichev, V. Shevchenko, V.

Elife. 2017 Jan 9;6. pii: e20954. doi: 10.7554/eLife.20954. [Epub ahead of print] PMID: 28067619

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology.

Henrich E, Peetz O, Hein C, Laguerre A, Hoffmann B, Hoffmann J, Dötsch V, Bernhard F, Morgner N. Elife. 2017 Jan 9;6. pii: e20954. doi: 10.7554/eLife.20954. [Epub ahead of print] PMID: 28067619

Details

Biopolymers. 2016 Dec 30. doi: 10.1002/bip.23013. [Epub ahead of print] PMID: 28035667

Acceleration of protein backbone NMR assignment by combinatorial labeling: Application to a small molecule binding study.

Hein C, Löhr F, Schwarz D, Dötsch V.

Details

EMBO J. 2016 Dec 27. pii: e201695063. doi: 10.15252/embj.201695063. [Epub ahead of print] PMID: 28028054

Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins.

Stolz A, Putyrski M, Kutle I, Huber J, Wang C, Major V, Sidhu SS, Youle RJ, Rogov VV, Dötsch V, Ernst A, Dikic I.

Details

J Neurochem. 2016 Nov 5. doi: 10.1111/jnc.13886. [Epub ahead of print] PMID: 27917477

The synaptic vesicle protein SV31 assembles into a dimer and transports Zn2.

Waberer L, Henrich E, Peetz O, Morgner N, Dötsch V, Bernhard F, Volknandt W.

Details

Cell Death Differ. 2016 Oct 7. doi: 10.1038/cdd.2016.83.

Mechanism of TAp73 inhibition by ΔNp63 and structural basis of p63/p73 hetero-tetramerization.

Gebel J, Luh LM, Coutandin D, Osterburg C, Löhr F, Schäfer B, Frombach AS, Sumyk M, Buchner L, Krojer T, Salah E, Mathea S, Güntert P, Knapp S, Dötsch V.

Details

Structure. 2016 Sep 15. pii: S0969-2126(16)30228-3. doi: 10.1016/j.str.2016.07.017. [Epub ahead of print]

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins.

Acceleration of protein backbone NMR assignment by combinatorial labeling: Application to a small molecule binding study. Hein C, Löhr F, Schwarz D, Dötsch V. Biopolymers. 2016 Dec 30. doi: 10.1002/bip.23013. [Epub ahead of print] PMID: 28035667 Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins. Stolz A, Putyrski M, Kutle I, Huber J, Wang C, Major V, Sidhu SS, Youle RJ, Rogov VV, Dötsch V, Ernst A, Dikic I. EMBO J. 2016 Dec 27. pii: e201695063. doi: 10.15252/embj.201695063. [Epub ahead of print] PMID: 28028054 The synaptic vesicle protein SV31 assembles into a dimer and transports Zn2. Waberer L, Henrich E, Peetz O, Morgner N, Dötsch V, Bernhard F, Volknandt W. J Neurochem. 2016 Nov 5. doi: 10.1111/jnc.13886. [Epub ahead of print] PMID: 27917477 Mechanism of TAp73 inhibition by ΔNp63 and structural basis of p63/p73 hetero-tetramerization. Gebel J, Luh LM, Coutandin D, Osterburg C, Löhr F, Schäfer B, Frombach AS, Sumyk M, Buchner L, Krojer T, Salah E, Mathea S, Güntert P, Knapp S, Dötsch V. Cell Death Differ. 2016 Oct 7. doi: 10.1038/cdd.2016.83. From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins. Laguerre A, Löhr F, Henrich E, Hoffmann B, Abdul-Manan N, Connolly PJ, Perozo E, Moore JM,Bernhard F, Dötsch V.

Details

Methods Mol Biol. 2016;1432:1-21. doi: 10.1007/978-1-4939-3637-3_1.

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies.

Rues RB, Henrich E, Boland C, Caffrey M, Bernhard F.

Details

ell Death Differ. 2016 Jul 22. doi: 10.1038/cdd.2016.75. [Epub ahead of print]

Intrinsic aggregation propensity of the p63 and p73 TI domains correlates with p53R175H interaction and suggests further significance of aggregation events in the p53 family.

Kehrloesser S, Osterburg C, Tuppi M, Schäfer B, Vousden KH, Dötsch V.

Details

Biochemistry. 2016 Jul 6. [Epub ahead of print] PMID: 27384110

Combining In vitro Folding with Cell-Free Protein Synthesis for Membrane Protein Expression.

Focke PJ, Hein C, Hoffmann B, Matulef K, Bernhard F, Dötsch V, Valiyaveetil FI.

Details

Mol Cell. 2016 May 31. pii: S1097-2765(16)30107-1. doi: 10.1016/j.molcel.2016.04.031. [Epub ahead of print]

The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation.

von Delbrück M, Kniss A, Rogov VV, Pluska L, Bagola K, Löhr F, Güntert P, Sommer T, Dötsch V.

Details

Biochim Biophys Acta. 2016 Jun;1858(6):1306-16. doi: 10.1016/j.bbamem.2016.02.031. Epub 2016 Feb 26.

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments.

Rues RB, Dötsch V, Bernhard F.

Details

Elife. 2016 Mar 29;5. pii: e13909. doi: 10.7554/eLife.13909. [Epub ahead of print]

Quality control in oocytes by p63 is based on a spring-loaded activation mechanism on the molecular and cellular level.

Coutandin D, Osterburg C, Srivastav RK, Sumyk M, Kehrloesser S, Gebel J, Tuppi M, Hannewald J, Schäfer B, Salah E, Mathea S, Müller-Kuller U, Doutch J, Grez M, Knapp S, Dötsch V.

Details

Cold Spring Harb Perspect Med. 2016 Apr 18. pii: a028308. doi: 10.1101/cshperspect.a028308. [Epub ahead of print]

Structural Evolution and Dynamics of the p53 Proteins.

Chillemi G, Kehrloesser S, Bernassola F, Desideri A, Dötsch V, Levine AJ, Melino G.

Details

J Biol Chem. 2016 Feb 29. pii: jbc.M116.715474. [Epub ahead of print]

Structural and functional analysis of a novel interaction motif within UFM1-activating enzyme 5 (UBA5) required for binding to ubiquitin-like proteins and ufmylation.

Henrich E, Ma Y, Engels I, Münch D, Otten C, Schneider T, Henrichfreise B, Sahl HG, Dötsch V, Bernhard F.

Details

Autophagy. 2016 Jan 2;12(1):1-222. No abstract available.

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).

Klionsky DJ, [...] Doetsch V, [...] Rogov VV et al.

Details

Methods Enzymol. 2015;565:367-88. doi: 10.1016/bs.mie.2015.06.001. Epub 2015 Jul 2.

Labeling of Membrane Proteins by Cell-Free Expression.

Laguerre A, Löhr F, Bernhard F, Dötsch V.

Details

Protein Sci 2015 Oct 16. doi: 10.1002/pro.2830. [Epub ahead of print]

Structural investigations of the p53/p73 homologs from the tunicate species Ciona intestinalis reveal the sequence requirements for the formation of a tetramerization domain.

Heering J, Jonker HR, Löhr F, Schwalbe H, Dötsch V.

Details

Bioorg Med Chem Lett. 2015 Nov 15;25(22):5243-6. doi: 10.1016/j.bmcl.2015.09.056. Epub 2015 Sep 28.

Probing metallo-β-lactamases with molecular fragments identified by consensus docking.

Klingler FM, Moser D, Büttner D, Wichelhaus TA, Löhr F, Dötsch V, Proschak E.

Details

Mol Cell. 2015 Oct 1;60(1):89-104. doi: 10.1016/j.molcel.2015.09.010.

TECPR2 Cooperates with LC3C to Regulate COPII-Dependent ER Export.

Stadel D, Millarte V, Tillmann KD, Huber J, Tamin-Yecheskel BC, Akutsu M, Demishtein A, Ben-Zeev B, Anikster Y, Perez F, Dötsch V, Elazar Z, Rogov V, Farhan H, Behrends C.

Details

J Biol Chem. 2015 Nov 13;290(46):27712-22. doi: 10.1074/jbc.M115.681205. Epub 2015 Sep 24.

Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing.

Mehler M, Eckert CE, Busche A, Kulhei J, Michaelis J, Becker-Baldus J, Wachtveitl J, Dötsch V, Glaubitz C.

Details

Biol Chem. 2015 Sep;396(9-10):1097-107. doi: 10.1515/hsz-2015-0105.

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing.

Merk H, Rues RB, Gless C, Beyer K, Dong F, Dötsch V, Gerrits M, Bernhard F.

Details

J Biol Chem. 2015 Jun 26;290(26):16415-30. doi: 10.1074/jbc.M115.660829. Epub 2015 May 15.

Structure and Biophysical Characterization of the S-Adenosylmethionine-dependent O-Methyltransferase PaMTH1, a Putative Enzyme Accumulating during Senescence of Podospora anserina.

Chatterjee D, Kudlinzki D, Linhard V, Saxena K, Schieborr U, Gande SL, Wurm JP, Wöhnert J, Abele R, Rogov VV, Dötsch V, Osiewacz HD, Sreeramulu S, Schwalbe H.

Details

J Biomol NMR. 2015 Jul;62(3):263-79. doi: 10.1007/s10858-015-9941-8. Epub 2015 May 8.

An extended combinatorial 15N, 13Cα, and 13C’ labeling approach to protein backbone resonance assignment.

Löhr F, Tumulka F, Bock C, Abele R, Dötsch V.

Details

FEBS Lett. 2015 Jul 8;589(15):1713-22. doi: 10.1016/j.febslet.2015.04.045. Epub 2015 May 1. Review.

Membrane protein production in Escherichia coli cell-free lysates.

Henrich E, Hein C, Dötsch V, Bernhard F.

Details

Methods Enzymol. 2015;556:351-69. doi: 10.1016/bs.mie.2014.12.016. Epub 2015 Mar 20.

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs.

Henrich E, Dötsch V, Bernhard F.

Details

Int J Cancer. 2015 Sep 15;137(6):1318-29. doi: 10.1002/ijc.29498. Epub 2015 Mar 12.

FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors.

Schwamb B, Pick R, Fernández SB, Völp K, Heering J, Dötsch V, Bösser S, Jung J, Beinoraviciute-Kellner R, Wesely J, Zörnig I, Hammerschmidt M, Nowak M, Penzel R, Zatloukal K, Joos S, Rieker RJ, Agaimy A, Söder S, Reid-Lombardo KM, Kendrick ML, Bardsley MR, Hayashi Y, Asuzu DT, Syed SA, Ordog T, Zörnig M.

Details

Mol Cell. 2015 Mar 19;57(6):995-1010. doi: 10.1016/j.molcel.2014.12.040. Epub 2015 Feb 12.

CUL3-KBTBD6/KBTBD7 ubiquitin ligase cooperates with GABARAP proteins to spatially restrict TIAM1-RAC1 signaling.

Genau HM, Huber J, Baschieri F, Akutsu M, Dötsch V, Farhan H, Rogov V, Behrends C.

Details

Methods Mol Biol. 2015;1261:171-95. doi: 10.1007/978-1-4939-2230-7_10.

Cell-free expression of G-protein-coupled receptors.

Orbán E, Proverbio D, Haberstock S, Dötsch V, Bernhard F.

Details

Methods Mol Biol. 2015;1258:125-43. doi: 10.1007/978-1-4939-2205-5_7. Review.

Co-translational stabilization of insoluble proteins in cell-free expression systems.

Kai L, Orbán E, Henrich E, Proverbio D, Dötsch V, Bernhard F.

Details

Oncogene. 2015 Aug 13;34(33):4300-10. doi: 10.1038/onc.2014.359. Epub 2014 Nov 24.

Functional interplay between MDM2, p63/p73 and mutant p53.

Stindt MH, Muller PA, Ludwig RL, Kehrloesser S, Dötsch V, Vousden KH.

Details

J Magn Reson. 2014 Nov;248:81-95. doi: 10.1016/j.jmr.2014.09.014. Epub 2014 Sep 30.

Time-shared experiments for efficient assignment of triple-selectively labeled proteins.

Löhr F, Laguerre A, Bock C, Reckel S, Connolly PJ, Abdul-Manan N, Tumulka F, Abele R, Moore JM, Dötsch V.

Details

Biol Chem. 2014 Dec;395(12):1425-34. doi: 10.1515/hsz-2014-0217. Review.

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets.

Rues RB, Orbán E, Dötsch V, Bernhard F.

Details

Biochim Biophys Acta. 2014 Oct;1842(10):1538-47. doi: 10.1016/j.bbalip.2014.07.022. Epub 2014 Aug 8.

Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase.

Schröder M, Häfner AK, Hofmann B, Rådmark O, Tumulka F, Abele R, Dötsch V, Steinhilber D.

Details

Angew Chem Int Ed Engl. 2014 Sep 22;53(39):10300-14. doi: 10.1002/anie.201311320. Epub 2014 Jul 28. Review.

In-cell NMR and EPR spectroscopy of biomacromolecules.

Hänsel R, Luh LM, Corbeski I, Trantirek L, Dötsch V.

Details

Cell Mol Life Sci. 2014 Dec;71(24):4895-910. doi: 10.1007/s00018-014-1655-7. Epub 2014 Jul 11.

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination.

Boland C, Li D, Shah ST, Haberstock S, Dötsch V, Bernhard F, Caffrey M.

Details

Chem Biol. 2014 Apr 24;21(4):552-62. doi: 10.1016/j.chembiol.2014.02.014. Epub 2014 Apr 3.

Crystal structure of a PCP/Sfp complex reveals the structural basis for carrier protein posttranslational modification.

Tufar P, Rahighi S, Kraas FI, Kirchner DK, Löhr F, Henrich E, Köpke J, Dikic I, Güntert P, Marahiel MA, Dötsch V.

Details

BMC Bioinformatics. 2014 Feb 11;15:46. doi: 10.1186/1471-2105-15-46.

Peak picking NMR spectral data using non-negative matrix factorization.

Tikole S, Jaravine V, Rogov V, Dötsch V, Güntert P.

Details

Mol Cell. 2014 Jan 23;53(2):167-78. doi: 10.1016/j.molcel.2013.12.014. Review.

Interactions between autophagy receptors and ubiquitin-like proteins form the molecular basis for selective autophagy.

Rogov V, Dötsch V, Johansen T, Kirkin V.

Details

Methods Mol Biol. 2014;1118:109-30. doi: 10.1007/978-1-62703-782-2_7.

High-level cell-free production of membrane proteins with nanodiscs.

Roos C, Kai L, Haberstock S, Proverbio D, Ghoshdastider U, Ma Y, Filipek S, Wang X, Dötsch V, Bernhard F.

Details

PLoS One. 2013 Sep 2;8(9):e72925. doi: 10.1371/journal.pone.0072925. eCollection 2013.

SPLICEFINDER – a fast and easy screening method for active protein trans-splicing positions.

Zettler J, Eppmann S, Busche A, Dikovskaya D, Dötsch V, Mootz HD, Sonntag T.

Details

J Biomol NMR. 2013 Oct;57(2):141-54. doi: 10.1007/s10858-013-9774-2. Epub 2013 Sep 7.

Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.

Tumulka F, Roos C, Löhr F, Bock C, Bernhard F, Dötsch V, Abele R.

Details

J Am Chem Soc. 2013 Sep 18;135(37):13796-803. doi: 10.1021/ja405244v. Epub 2013 Sep 6.

Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition.

Luh LM, Hänsel R, Löhr F, Kirchner DK, Krauskopf K, Pitzius S, Schäfer B, Tufar P, Corbeski I, Güntert P, Dötsch V.

Details

Elife. 2013 Jul 30;2:e01159. doi: 10.7554/eLife.01159.

How to switch a master switch.

Hein C, Wittinghofer A, Dötsch V.

Details

Cold Spring Harb Perspect Biol. 2013 Aug 1;5(8):a013334. doi: 10.1101/cshperspect.a013334. Review.

Endoplasmic reticulum targeting and insertion of tail-anchored membrane proteins by the GET pathway.

Denic V, Dötsch V, Sinning I.

Details

Biochem J. 2013 Sep 15;454(3):459-66.

Structural basis for phosphorylation-triggered autophagic clearance of Salmonella.

Proverbio D, Roos C, Beyermann M, Orbán E, Dötsch V, Bernhard F.

Details

Cell Death Differ. 2013 Aug;20(8):1008-16. doi: 10.1038/cdd.2013.23. Epub 2013 Mar 29.

Analysis of the oligomeric state and transactivation potential of TAp73α.

Luh LM, Kehrloesser S, Deutsch GB, Gebel J, Coutandin D, Schäfer B, Agostini M, Melino G, Dötsch V.

Details

PLoS One. 2013;8(2):e56637. doi: 10.1371/journal.pone.0056637. Epub 2013 Feb 22.

Artificial environments for the co-translational stabilization of cell-free expressed proteins.

Hänsel R, Löhr F, Trantirek L, Dötsch V.

Details

Biochim Biophys Acta. 2013 Apr;1828(4):1222-9. doi: 10.1016/j.bbamem.2012.12.011. Epub 2012 Dec 28.

Modified lipid and protein dynamics in nanodiscs.

Mörs K, Roos C, Scholz F, Wachtveitl J, Dötsch V, Bernhard F, Glaubitz C.

Details

Biomol NMR. 2012 Dec;54(4):401-13. doi: 10.1007/s10858-012-9684-8. Epub 2012 Nov 17.

Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies.

Stehle J, Scholz F, Löhr F, Reckel S, Roos C, Blum M, Braun M, Glaubitz C, Dötsch V, Wachtveitl J, Schwalbe H.

Details

Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies. Stehle J, Scholz F, Löhr F, Reckel S, Roos C, Blum M, Braun M, Glaubitz C, Dötsch V, Wachtveitl J, Schwalbe H. J Biomol NMR. 2012 Dec;54(4):401-13. doi: 10.1007/s10858-012-9684-8. Epub 2012 Nov 17. A method for integrative structure determination of protein-protein complexes. Schneidman-Duhovny D, Rossi A, Avila-Sakar A, Kim SJ, Velázquez-Muriel J, Strop P, Liang H, Krukenberg KA, Liao M, Kim HM, Sobhanifar S, Dötsch V, Rajpal A, Pons J, Agard DA, Cheng Y, Sali A. Bioinformatics. 2012 Dec 15;28(24):3282-9. doi: 10.1093/bioinformatics/bts628. Epub 2012 Oct 23.

A method for integrative structure determination of protein-protein complexes.

Schneidman-Duhovny D, Rossi A, Avila-Sakar A, Kim SJ, Velázquez-Muriel J, Strop P, Liang H, Krukenberg KA, Liao M, Kim HM, Sobhanifar S, Dötsch V, Rajpal A, Pons J, Agard DA, Cheng Y, Sali A.

Details

Protein Expr Purif. 2012 Dec;86(2):120-6. doi: 10.1016/j.pep.2012.09.011. Epub 2012 Oct 2.

Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening.

Ma Y, Ghoshdastider U, Wang J, Ye W, Dötsch V, Filipek S, Bernhard F, Wang X.

Details

Biochim Biophys Acta. 2012 Dec;1818(12):3098-106. doi: 10.1016/j.bbamem.2012.08.007. Epub 2012 Aug 18.

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.

Roos C, Zocher M, Müller D, Münch D, Schneider T, Sahl HG, Scholz F, Wachtveitl J, Ma Y, Proverbio D, Henrich E, Dötsch V, Bernhard F.

Details

Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15312-7. Epub 2012 Sep 4. Erratum in: Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2855.

Loss of p63 and its microRNA-205 target results in enhanced cell migration and metastasis in prostate cancer.

Tucci P, Agostini M, Grespi F, Markert EK, Terrinoni A, Vousden KH, Muller PA, Dötsch V, Kehrloesser S, Sayan BS, Giaccone G, Lowe SW, Takahashi N, Vandenabeele P, Knight RA, Levine AJ, Melino G.

Details

Top Curr Chem. 2013;330:47-65. doi: 10.1007/128_2012_332. Review.

Investigation of quadruplex structure under physiological conditions using in-cell NMR.

Hänsel R, Foldynová-Trantírková S, Dötsch V, Trantírek L.

Details

Mol Membr Biol. 2013 Feb;30(1):75-89. doi: 10.3109/09687688.2012.693212. Epub 2012 Jun 20.

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers.

Roos C, Kai L, Proverbio D, Ghoshdastider U, Filipek S, Dötsch V, Bernhard F.

Details

Structure. 2012 Jun 6;20(6):1019-27. doi: 10.1016/j.str.2012.03.010. Epub 2012 May 3.

Requirements on paramagnetic relaxation enhancement data for membrane protein structure determination by NMR.

Gottstein D, Reckel S, Dötsch V, Güntert P.

Details

EMBO J. 2012 May 30;31(11):2615-28. doi: 10.1038/emboj.2012.93. Epub 2012 Apr 24.

Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis.

Imre G, Heering J, Takeda AN, Husmann M, Thiede B, zu Heringdorf DM, Green DR, van der Goot FG, Sinha B, Dötsch V, Rajalingam K.

Details

Chembiochem. 2012 May 7;13(7):964-7. doi: 10.1002/cbic.201200044. Epub 2012 Apr 11.

Fast automated NMR spectroscopy of short-lived biological samples.

Tikole S, Jaravine V, Rogov VV, Rozenknop A, Schmöe K, Löhr F, Dötsch V, Güntert P.

Details

Chembiochem. 2012 May 7;13(7):959-63. doi: 10.1002/cbic.201200045. Epub 2012 Mar 20.

A universal expression tag for structural and functional studies of proteins.

Rogov VV, Rozenknop A, Rogova NY, Löhr F, Tikole S, Jaravine V, Güntert P, Dikic I, Dötsch V.

Details

Protein Expr Purif. 2012 Apr;82(2):308-16. doi: 10.1016/j.pep.2012.01.018. Epub 2012 Feb 8.

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.

Haberstock S, Roos C, Hoevels Y, Dötsch V, Schnapp G, Pautsch A, Bernhard F.

Details

Chembiochem. 2012 Mar 5;13(4):534-7. doi: 10.1002/cbic.201100721. Epub 2012 Feb 1.

In-cell solid-state NMR as a tool to study proteins in large complexes.

Reckel S, Lopez JJ, Löhr F, Glaubitz C, Dötsch V.

Details

J Biomol NMR. 2012 Mar;52(3):197-210. doi: 10.1007/s10858-012-9601-1. Epub 2012 Jan 18.

Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment.

Löhr F, Reckel S, Karbyshev M, Connolly PJ, Abdul-Manan N, Bernhard F, Moore JM, Dötsch V.

Details

ACS Nano. 2012 Jan 24;6(1):961-71. doi: 10.1021/nn204624p. Epub 2011 Dec 30.

Single-molecule force spectroscopy from nanodiscs: an assay to quantify folding, stability, and interactions of native membrane proteins.

Zocher M, Roos C, Wegmann S, Bosshart PD, Dötsch V, Bernhard F, Müller DJ.

Details

ACS Chem Biol. 2012 Feb 17;7(2):378-86. doi: 10.1021/cb200352q. Epub 2011 Dec 15.

Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthase.

usche A, Gottstein D, Hein C, Ripin N, Pader I, Tufar P, Eisman EB, Gu L, Walsh CT, Sherman DH, Löhr F, Güntert P, Dötsch V.

Details

Angew Chem Int Ed Engl. 2011 Dec 9;50(50):11942-6. doi: 10.1002/anie.201105648. Epub 2011 Oct 27.

Solution NMR structure of proteorhodopsin.

Reckel S, Gottstein D, Stehle J, Löhr F, Verhoefen MK, Takeda M, Silvers R, Kainosho M, Glaubitz C, Wachtveitl J, Bernhard F, Schwalbe H, Güntert P, Dötsch V.

Details

Methods Mol Biol. 2012;800:201-25. doi: 10.1007/978-1-61779-349-3_14.

Systems for the cell-free synthesis of proteins.

Kai L, Roos C, Haberstock S, Proverbio D, Ma Y, Junge F, Karbyshev M, Dötsch V, Bernhard F.

Details

J Biol Chem. 2011 Nov 11;286(45):38844-53. doi: 10.1074/jbc.M111.301085. Epub 2011 Sep 20.

Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes.

Ma Y, Münch D, Schneider T, Sahl HG, Bouhss A, Ghoshdastider U, Wang J, Dötsch V, Wang X, Bernhard F.

Details

J Mol Biol. 2011 Oct 28;413(3):593-603. doi: 10.1016/j.jmb.2011.08.055. Epub 2011 Sep 10.

Cell-free expression and assembly of ATP synthase.

Matthies D, Haberstock S, Joos F, Dötsch V, Vonck J, Bernhard F, Meier T.

Details

J Biol Chem. 2011 Oct 28;286(43):37874-86. doi: 10.1074/jbc.M111.289330. Epub 2011 Sep 6.

The large extracellular loop of organic cation transporter 1 influences substrate affinity and is pivotal for oligomerization.

Keller T, Egenberger B, Gorboulev V, Bernhard F, Uzelac Z, Gorbunov D, Wirth C, Koppatz S, Dötsch V, Hunte C, Sitte HH, Koepsell H.

Details

J Biol Chem. 2011 Sep 16;286(37):32525-32. doi: 10.1074/jbc.M111.276956. Epub 2011 Jul 28.

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification.

Rath P, Demange P, Saurel O, Tropis M, Daffé M, Dötsch V, Ghazi A, Bernhard F, Milon A.

Details

Science. 2011 Aug 5;333(6043):758-62. doi: 10.1126/science.1207125. Epub 2011 Jun 30.

Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex.

Stefer S, Reitz S, Wang F, Wild K, Pang YY, Schwarz D, Bomke J, Hein C, Löhr F, Bernhard F, Denic V, Dötsch V, Sinning I.

Details

Cell Sci. 2011 Jul 1;124(Pt 13):2200-7. doi: 10.1242/jcs.079327. Epub 2011 Jun 7.

Differential altered stability and transcriptional activity of ΔNp63 mutants in distinct ectodermal dysplasias.

Browne G, Cipollone R, Lena AM, Serra V, Zhou H, van Bokhoven H, Dötsch V, Merico D, Mantovani R, Terrinoni A, Knight RA, Candi E, Melino G.

Details

J Biomol NMR. 2011 Jun;50(2):167-90. doi: 10.1007/s10858-011-9507-3. Epub 2011 Jun 7.

Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (α) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy.

Löhr F, Reckel S, Stefer S, Dötsch V, Schmidt JM.

Details

J Mol Biol. 2011 Jul 15;410(3):477-87. doi: 10.1016/j.jmb.2011.05.003. Epub 2011 May 18.

Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1.

Rozenknop A, Rogov VV, Rogova NY, Löhr F, Güntert P, Dikic I, Dötsch V.

Details

Science. 2011 Jul 8;333(6039):228-33. doi: 10.1126/science.1205405. Epub 2011 May 26.

Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth.

Wild P, Farhan H, McEwan DG, Wagner S, Rogov VV, Brady NR, Richter B, Korac J, Waidmann O, Choudhary C, Dötsch V, Bumann D, Dikic I.

Details

Am J Med Genet A. 2011 Jun;155A(6):1432-6. doi: 10.1002/ajmg.a.34011. Epub 2011 May 12.

Mutation in SAM domain of TP63 is associated with nonsyndromic cleft lip and palate and cleft palate.

Kantaputra PN, Malaivijitnond S, Vieira AR, Heering J, Dötsch V, Khankasikum T, Sripathomsawat W.

Details

Structure. 2011 May 11;19(5):601-2. doi: 10.1016/j.str.2011.04.003.

How to create a specific recognition for an unspecific interaction.

Dötsch V.

Details

Cell Cycle. 2011 Jun 15;10(12):1884-5. Epub 2011 Jun 15.

Quality control in oocytes: domain-domain interactions regulate the activity of p63.

Deutsch GB, Zielonka EM, Coutandin D, Dötsch V.

Details

Angew Chem Int Ed Engl. 2011 May 23;50(22):5070-4. doi: 10.1002/anie.201100886. Epub 2011 Apr 19.

Long-range distance measurements on nucleic acids in cells by pulsed EPR spectroscopy.

Krstić I, Hänsel R, Romainczyk O, Engels JW, Dötsch V, Prisner TF.

Details

J Struct Biol. 2011 Jun;174(3):434-42. doi: 10.1016/j.jsb.2011.04.001. Epub 2011 Apr 9.

Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements.

Kodama Y, Reese ML, Shimba N, Ono K, Kanamori E, Dötsch V, Noguchi S, Fukunishi Y, Suzuki E, Shimada I, Takahashi H.

Details

Structure. 2011 Apr 13;19(4):577-87. doi: 10.1016/j.str.2011.01.012.

Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsB.

Schmöe K, Rogov VV, Rogova NY, Löhr F, Güntert P, Bernhard F, Dötsch V.

Details

J Biol Chem. 2011 May 27;286(21):18775-83. doi: 10.1074/jbc.M111.230185. Epub 2011 Apr 6.

A disulfide bridge network within the soluble periplasmic domain determines structure and function of the outer membrane protein RCSF.

Rogov VV, Rogova NY, Bernhard F, Löhr F, Dötsch V.

Details

Nucleic Acids Res. 2011 Jul;39(13):5768-75. doi: 10.1093/nar/gkr174. Epub 2011 Mar 30.

The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions.

Deutsch GB, Zielonka EM, Coutandin D, Weber TA, Schäfer B, Hannewald J, Luh LM, Durst FG, Ibrahim M, Hoffmann J, Niesen FH, Sentürk A, Kunkel H, Brutschy B, Schleiff E, Knapp S, Acker-Palmer A, Grez M, McKeon F, Dötsch V.

Details

Am J Med Genet A. 2011 Jan;155A(1):228-32. doi: 10.1002/ajmg.a.33768.

Phenotypic analysis of Arg227 mutations of TP63 with emphasis on dental phenotype and micturition difficulties in EEC syndrome.

Sripathomsawat W, Tanpaiboon P, Heering J, Dötsch V, Hennekam RC, Kantaputra P.

Details

J Biomol NMR. 2011 Feb;49(2):75-84. doi: 10.1007/s10858-010-9462-4. Epub 2010 Dec 18.

Optimization of amino acid type-specific 13C and 15N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm.

Hefke F, Bagaria A, Reckel S, Ullrich SJ, Dötsch V, Glaubitz C, Güntert P.

Details

PLoS One. 2010 Sep 24;5(9):e12972. doi: 10.1371/journal.pone.0012972.

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes.

Kai L, Kaldenhoff R, Lian J, Zhu X, Dötsch V, Bernhard F, Cen P, Xu Z.

Details

Biochemistry. 2010 Oct 19;49(41):8988-98. doi: 10.1021/bi100537f.

NmerA of Tn501 mercuric ion reductase: structural modulation of the pKa values of the metal binding cysteine thiols.

Ledwidge R, Hong B, Dötsch V, Miller SM.

Details

N Biotechnol. 2011 Apr 30;28(3):262-71. doi: 10.1016/j.nbt.2010.07.002. Epub 2010 Jul 15.

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins.

Junge F, Haberstock S, Roos C, Stefer S, Proverbio D, Dötsch V, Bernhard F.

Details

Cold Spring Harb Perspect Biol. 2010 Sep;2(9):a004887. doi: 10.1101/cshperspect.a004887. Epub 2010 May 19. Review.

p63 and p73, the ancestors of p53.

Dötsch V, Bernassola F, Coutandin D, Candi E, Melino G.

Details

J Struct Biol. 2010 Oct;172(1):94-106. doi: 10.1016/j.jsb.2010.05.004. Epub 2010 May 9.

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptor.

Junge F, Luh LM, Proverbio D, Schäfer B, Abele R, Beyermann M, Dötsch V, Bernhard F.

Details

Proc Natl Acad Sci U S A. 2010 May 25;107(21):9644-9. doi: 10.1073/pnas.1000778107. Epub 2010 May 5.

Structural investigation of the C-terminal catalytic fragment of presenilin 1.

Sobhanifar S, Schneider B, Löhr F, Gottstein D, Ikeya T, Mlynarczyk K, Pulawski W, Ghoshdastider U, Kolinski M, Filipek S, Güntert P, Bernhard F, Dötsch V.

Details

Methods Mol Biol. 2010;607:187-212. doi: 10.1007/978-1-60327-331-2_16.

Strategies for the cell-free expression of membrane proteins.

Reckel S, Sobhanifar S, Durst F, Löhr F, Shirokov VA, Dötsch V, Bernhard F.

Details

Proteomics. 2010 May;10(9):1762-79. doi: 10.1002/pmic.200900485.

Cell-free expression profiling of E. coli inner membrane proteins.

Schwarz D, Daley D, Beckhaus T, Dötsch V, Bernhard F.

Details

Methods Mol Biol. 2010;601:165-86. doi: 10.1007/978-1-60761-344-2_11.

Membrane protein expression in cell-free systems.

Schneider B, Junge F, Shirokov VA, Durst F, Schwarz D, Dötsch V, Bernhard F.

Details

Cell Death Dis. 2010;1:e5. doi: 10.1038/cddis.2009.1.

The C-terminus of p63 contains multiple regulatory elements with different functions.

Straub WE, Weber TA, Schäfer B, Candi E, Durst F, Ou HD, Rajalingam K, Melino G, Dötsch V.

Details

EMBO Rep. 2010 Jan;11(1):45-51. doi: 10.1038/embor.2009.256. Epub 2009 Dec 11.

Nix is a selective autophagy receptor for mitochondrial clearance.

Novak I, Kirkin V, McEwan DG, Zhang J, Wild P, Rozenknop A, Rogov V, Löhr F, Popovic D, Occhipinti A, Reichert AS, Terzic J, Dötsch V, Ney PA, Dikic I.

Details

Nat Struct Mol Biol. 2009 Dec;16(12):1209-10. doi: 10.1038/nsmb1209-1209.

Ubiquitin linkages make a difference.

Dikic I, Dötsch V.

Details

J Pharmacol Exp Ther. 2010 Mar;332(3):840-8. doi: 10.1124/jpet.109.160663. Epub 2009 Nov 24.

The molecular pharmacology and in vivo activity of 2-(4-chloro-6-(2,3-dimethylphenylamino)pyrimidin-2-ylthio)octanoic acid (YS121), a dual inhibitor of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase.

Koeberle A, Rossi A, Zettl H, Pergola C, Dehm F, Bauer J, Greiner C, Reckel S, Hoernig C, Northoff H, Bernhard F, Dötsch V, Sautebin L, Schubert-Zsilavecz M, Werz O.

Details

J Mol Biol. 2009 Dec 18;394(5):852-63. doi: 10.1016/j.jmb.2009.10.014. Epub 2009 Oct 22.

NMR studies reveal the role of biomembranes in modulating ligand binding and release by intracellular bile acid binding proteins.

Pedò M, Löhr F, D'Onofrio M, Assfalg M, Dötsch V, Molinari H.

Details

J Am Chem Soc. 2009 Nov 4;131(43):15761-8. doi: 10.1021/ja9052027.

Evaluation of parameters critical for observing nucleic acids inside living Xenopus laevis oocytes by in-cell NMR spectroscopy.

Hänsel R, Foldynová-Trantírková S, Löhr F, Buck J, Bongartz E, Bamberg E, Schwalbe H, Dötsch V, Trantírek L.

Details

Cell Death Differ. 2009 Dec;16(12):1582-9. doi: 10.1038/cdd.2009.139. Epub 2009 Sep 18.

Conformational stability and activity of p73 require a second helix in the tetramerization domain.

Coutandin D, Löhr F, Niesen FH, Ikeya T, Weber TA, Schäfer B, Zielonka EM, Bullock AN, Yang A, Güntert P, Knapp S, McKeon F, Ou HD, Dötsch V.

Details

EMBO Rep. 2009 Sep;10(9):936-7. doi: 10.1038/embor.2009.190.

On track to tenure-track.

Dötsch V.

Details

J Biomol NMR. 2010 Jan;46(1):33-43. doi: 10.1007/s10858-009-9364-5. Epub 2009 Aug 13.

Cell-free expression and stable isotope labelling strategies for membrane proteins.

Sobhanifar S, Reckel S, Junge F, Schwarz D, Kai L, Karbyshev M, Löhr F, Bernhard F, Dötsch V.

Details

Angew Chem Int Ed Engl. 2009;48(33):6128-31. doi: 10.1002/anie.200901488.

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

Busche AE, Aranko AS, Talebzadeh-Farooji M, Bernhard F, Dötsch V, Iwaï H.

Details

J Biomol Struct Dyn. 2009 Jun;26(6):755-61.

A cost-effective amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae.

Foldynová-Trantirková S, Matulová J, Dötsch V, Löhr F, Cirstea I, Alexandov K, Breitling R, Lukes J, Trantírek L.

Details

Biochem Soc Trans. 2008 Dec;36(Pt 6):1427-32. doi: 10.1042/BST0361427.

Modulation of the Rcs-mediated signal transfer by conformational flexibility.

Rogov VV, Schmöe K, Löhr F, Rogova NY, Bernhard F, Dötsch V.

Details

J Am Chem Soc. 2008 Nov 12;130(45):14932-3. doi: 10.1021/ja806212j. Epub 2008 Oct 21.

The better tag remains unseen.

Durst FG, Ou HD, Löhr F, Dötsch V, Straub WE.

Details

Proteomics. 2008 Oct;8(19):3933-46. doi: 10.1002/pmic.200800171. Review.

Production of membrane proteins using cell-free expression systems.

Schwarz D, Dötsch V, Bernhard F.

Details

Nature. 2008 Aug 14;454(7206):907-11. doi: 10.1038/nature07161.

Structural basis for the selectivity of the external thioesterase of the surfactin synthetase.

Koglin A, Löhr F, Bernhard F, Rogov VV, Frueh DP, Strieter ER, Mofid MR, Güntert P, Wagner G, Walsh CT, Marahiel MA, Dötsch V.

Details

Proc Natl Acad Sci U S A. 2008 Jun 17;105(24):8262-7. doi: 10.1073/pnas.0710843105. Epub 2008 Jun 11.

Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins.

Reckel S, Sobhanifar S, Schneider B, Junge F, Schwarz D, Durst F, Löhr F, Güntert P, Bernhard F, Dötsch V.

Details

Cell Mol Life Sci. 2008 Jun;65(11):1729-55. doi: 10.1007/s00018-008-8067-5. Review.

Large-scale production of functional membrane proteins.

Junge F, Schneider B, Reckel S, Schwarz D, Dötsch V, Bernhard F.

Details

Biochemistry. 2008 Apr 15;47(15):4552-64. doi: 10.1021/bi800060w. Epub 2008 Mar 25.

Cell free expression and functional reconstitution of eukaryotic drug transporters.

Keller T, Schwarz D, Bernhard F, Dötsch V, Hunte C, Gorboulev V, Koepsell H.

Details

Oncogene. 2008 Jun 12;27(26):3739-45. doi: 10.1038/sj.onc.1211042. Epub 2008 Jan 21.

Ubiquitin binding mediates the NF-kappaB inhibitory potential of ABIN proteins.

Wagner S, Carpentier I, Rogov V, Kreike M, Ikeda F, Löhr F, Wu CJ, Ashwell JD, Dötsch V, Dikic I, Beyaert R.

Details

Top Curr Chem. 2008;273:203-14. doi: 10.1007/128_2007_21.

Investigation of proteins in living bacteria with in-cell NMR experiments.

Dötsch V.

Details

EMBO J. 2007 Jul 25;26(14):3451-62. Epub 2007 Jun 28.

Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genes.

Ikeda F, Hecker CM, Rozenknop A, Nordmeier RD, Rogov V, Hofmann K, Akira S, Dötsch V, Dikic I.

Details

Mol Cell. 2007 Jun 22;26(6):891-8.

E3-independent monoubiquitination of ubiquitin-binding proteins.

Hoeller D, Hecker CM, Wagner S, Rogov V, Dötsch V, Dikic I.

Details

EMBO J. 2007 Jul 25;26(14):3463-73. Epub 2007 Jun 21.

Structural evolution of C-terminal domains in the p53 family.

Ou HD, Löhr F, Vogel V, Mäntele W, Dötsch V.

Details

J Struct Biol. 2007 Jun;158(3):482-93. Epub 2007 Jan 23.

Cell-free production of G protein-coupled receptors for functional and structural studies.

Klammt C, Schwarz D, Eifler N, Engel A, Piehler J, Haase W, Hahn S, Dötsch V, Bernhard F.

Details

J Biomol NMR. 2007 Mar;37(3):205-24. Epub 2007 Jan 20.

Improved pulse sequences for sequence specific assignment of aromatic proton resonances in proteins.

Löhr F, Hänsel R, Rogov VV, Dötsch V.

Details

Nat Struct Mol Biol. 2007 Feb;14(2):155-63. Epub 2007 Jan 14.

The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a.

Reese ML, Dakoji S, Bredt DS, Dötsch V.

Details

J Biomol NMR. 2007 Jun;38(2):165. Epub 2006 Oct 12.

NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain.

Rogov VV, Löhr F, Rogova N, Klammt C, Koglin A, Bernhard F, Dötsch V.

Details

Methods. 2007 Apr;41(4):355-69. Review.

Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies.

Schwarz D, Klammt C, Koglin A, Löhr F, Schneider B, Dötsch V, Bernhard F.

Details

Nat Protoc. 2007;2(11):2945-57.

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.

Schwarz D, Junge F, Durst F, Frölich N, Schneider B, Reckel S, Sobhanifar S, Dötsch V, Bernhard F.

Details

J Struct Biol. 2007 Aug;159(2):194-205.

Reprint of “Cell-free production of G protein-coupled receptors for functional and structural studies” [J. Struct. Biol. 158 (2007) 482-493].

Klammt C, Schwarz D, Dötsch V, Bernhard F.

Details

Nat Protoc. 2006;1(6):2701-9.

Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy.

Serber Z, Selenko P, Hänsel R, Reckel S, Löhr F, Ferrell JE Jr, Wagner G, Dötsch V.

Details

J Mol Biol. 2006 Nov 17;364(1):68-79. Epub 2006 Jul 29.

A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.

Rogov VV, Rogova NY, Bernhard F, Koglin A, Löhr F, Dötsch V.

Details

FEBS J. 2006 Sep;273(18):4141-53. Epub 2006 Aug 23. Review.

Cell-free expression as an emerging technique for the large scale production of integral membrane protein.

Klammt C, Schwarz D, Löhr F, Schneider B, Dötsch V, Bernhard F.

Details

Magn Reson Chem. 2006 Jul;44 Spec No:S17-23. Review.

Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins.

Koglin A, Klammt C, Trbovic N, Schwarz D, Schneider B, Schäfer B, Löhr F, Bernhard F, Dötsch V.

Details

J Biomol NMR. 2006;36 Suppl 1:15.

NMR assignment of the L27 heterodimer from LIN-2 and LIN-7 scaffold proteins.

Petrosky KY, Löhr F, Dötsch V.

Details

Science. 2006 Apr 14;312(5771):273-6.

Conformational switches modulate protein interactions in peptide antibiotic synthetases.

Koglin A, Mofid MR, Löhr F, Schäfer B, Rogov VV, Blum MM, Mittag T, Marahiel MA, Bernhard F, Dötsch V.

Details

Biochemistry. 2006 Mar 21;45(11):3572-9.

One functional switch mediates reversible and irreversible inactivation of a herpesvirus protease.

Nomura AM, Marnett AB, Shimba N, Dötsch V, Craik CS.

Details

J Biol Chem. 2006 Apr 14;281(15):10589-97. Epub 2005 Dec 23.

Actin binding to the central domain of WASP/Scar proteins plays a critical role in the activation of the Arp2/3 complex.

Kelly AE, Kranitz H, Dötsch V, Mullins RD.

Details

FEBS J. 2005 Dec;272(23):6024-38.

Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system.

Klammt C, Schwarz D, Fendler K, Haase W, Dötsch V, Bernhard F.

Details

Nat Struct Mol Biol. 2005 Nov;12(11):1019-20.

Induced structure of a helical switch as a mechanism to regulate enzymatic activity.

Nomura AM, Marnett AB, Shimba N, Dötsch V, Craik CS.

Details

J Biomol NMR. 2005 Aug;32(4):309-28.

Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues.

Löhr F, Rogov VV, Shi M, Bernhard F, Dötsch V.

Details

J Am Chem Soc. 2005 Oct 5;127(39):13504-5.

Efficient strategy for the rapid backbone assignment of membrane proteins.

Trbovic N, Klammt C, Koglin A, Löhr F, Bernhard F, Dötsch V.

Details

J Biol Chem. 2005 Nov 18;280(46):38528-36. Epub 2005 Sep 7.

A general model for preferential hetero-oligomerization of LIN-2/7 domains: mechanism underlying directed assembly of supramolecular signaling complexes.

Petrosky KY, Ou HD, Löhr F, Dötsch V, Lim WA.

Details

Methods Enzymol. 2005;394:17-41.

In-cell NMR spectroscopy.

Serber Z, Corsini L, Durst F, Dötsch V.

Details

J Biomol NMR. 2004 Oct;30(2):175-9.

Optimization of 13C direct detection NMR methods.

Shimba N, Kovacs H, Stern AS, Nomura AM, Shimada I, Hoch JC, Craik CS, Dötsch V.

Details

J Mol Biol. 2004 Oct 29;343(4):1035-48.

Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains.

Rogov VV, Bernhard F, Löhr F, Dötsch V.

Details

J Biomol NMR. 2004 Sep;30(1):103-4.

Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain.

V Rogov V, Löhr F, Bernhard F, Dötsch V.

Details

J Am Chem Soc. 2004 Jun 9;126(22):7119-25.

Methyl groups as probes for proteins and complexes in in-cell NMR experiments.

Serber Z, Straub W, Corsini L, Nomura AM, Shimba N, Craik CS, Ortiz de Montellano P, Dötsch V.

Details

Eur J Biochem. 2004 Feb;271(3):568-80.

High level cell-free expression and specific labeling of integral membrane proteins.

Klammt C, Löhr F, Schäfer B, Haase W, Dötsch V, Rüterjans H, Glaubitz C, Bernhard F.

Details

J Am Chem Soc. 2003 Nov 26;125(47):14250-1. PMID: 14624553 [PubMed - indexed for MEDLINE]

Fast mapping of protein-protein interfaces by NMR spectroscopy.

Reese ML, Dötsch V.

Details

Biochemistry. 2003 Aug 5;42(30):9227-34.

Quantitative identification of the protonation state of histidines in vitro and in vivo.

Shimba N, Serber Z, Ledwidge R, Miller SM, Craik CS, Dötsch V.

Details

J Am Chem Soc. 2003 Mar 5;125(9):2382-3.

Elimination of 13Calpha splitting in protein NMR spectra by deconvolution with maximum entropy reconstruction.

Shimba N, Stern AS, Craik CS, Hoch JC, Dötsch V.

Details

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1150-61. Epub 2006 Sep 19. Review.

NMR in the SPINE Structural Proteomics project.

Ab E, Atkinson AR, Banci L, Bertini I, Ciofi-Baffoni S, Brunner K, Diercks T, Dötsch V, Engelke F, Folkers GE, Griesinger C, Gronwald W, Günther U, Habeck M, de Jong RN, Kalbitzer HR, Kieffer B, Leeflang BR, Loss S, Luchinat C, Marquardsen T, Moskau D, Neidig KP, Nilges M, Piccioli M, Pierattelli R, Rieping W, Schippmann T, Schwalbe H, Travé G, Trenner J, Wöhnert J, Zweckstetter M, Kaptein R.

Details

Mol Cell Biol. 2002 Dec;22(24):8601-11.

A C-terminal inhibitory domain controls the activity of p63 by an intramolecular mechanism.

Serber Z, Lai HC, Yang A, Ou HD, Sigal MS, Kelly AE, Darimont BD, Duijf PH, Van Bokhoven H, McKeon F, Dötsch V.

Details

Am Chem Soc. 2002 Oct 9;124(40):12013-9.

Low-conductivity buffers for high-sensitivity NMR measurements.

Kelly AE, Ou HD, Withers R, Dötsch V.

Details

Hum Mol Genet. 2002 Apr 1;11(7):799-804.

Gain-of-function mutation in ADULT syndrome reveals the presence of a second transactivation domain in p63.

Duijf PH, Vanmolkot KR, Propping P, Friedl W, Krieger E, McKeon F, Dötsch V, Brunner HG, van Bokhoven H.

Details

Chembiochem. 2001 Apr 2;2(4):247-51.

Carbon-detected NMR experiments to investigate structure and dynamics of biological macromolecules.

Serber Z, Richter C, Dötsch V.

Details

J Biomol NMR 2001 Nov;21(3):269-73.

Efficient identification of amino acid types for fast protein backbone assignments.

Ou HD, Lai HC, Serber Z, Dötsch V.

Details

J Am Chem Soc. 2001 Sep 19;123(37):8895-901.

Evaluation of parameters critical to observing proteins inside living Escherichia coli by in-cell NMR spectroscopy.

Serber Z, Ledwidge R, Miller SM, Dötsch V.

Details

Methods Enzymol. 2001;339:343-57. Review.

Protein-DNA interactions.

Dötsch V.

Details

J Am Chem Soc. 2001 Mar 14;123(10):2446-7.

High-resolution macromolecular NMR spectroscopy inside living cells.

Serber Z, Keatinge-Clay AT, Ledwidge R, Kelly AE, Miller SM, Dötsch V.

Details

Biochemistry. 2001 Apr 10;40(14):4261-71.

Structural basis for the functional switch of the E. coli Ada protein.

Lin Y, Dötsch V, Wintner T, Peariso K, Myers LC, Penner-Hahn JE, Verdine GL, Wagner G.

Details

Nat Struct Biol. 1999 Jul;6(7):656-60.

The GYF domain is a novel structural fold that is involved in lymphoid signaling through proline-rich sequences.

Freund C, Dötsch V, Nishizawa K, Reinherz EL, Wagner G.

Details

EMBO J. 1999 Jun 1;18(11):2941-9.

Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies.

Sun ZY, Dötsch V, Kim M, Li J, Reinherz EL, Wagner G.

Details

Curr Opin Struct Biol. 1998 Oct;8(5):619-23. Review.

New approaches to structure determination by NMR spectroscopy.

Dötsch V, Wagner G.

Details

Cell. 1998 Mar 6;92(5):687-96.

Solution structure of the core NFATC1/DNA complex.

Zhou P, Sun LJ, Dötsch V, Wagner G, Verdine GL.

Details

Nature. 1997 Jan 9;385(6612):172-6.

Unusual Rel-like architecture in the DNA-binding domain of the transcription factor NFATc.

Wolfe SA, Zhou P, Dötsch V, Chen L, You A, Ho SN, Crabtree GR, Wagner G, Verdine GL.

Details

Curr Opin Biotechnol. 1996 Aug;7(4):428-32. Review.

The role of protein-solvent interactions in protein unfolding.

Schiffer CA, Dötsch V.

Details

J Magn Reson B. 1996 Jul;112(1):95-100.

Amino-acid-type identification for deuterated proteins with a beta-carbon-edited HNCOCACB experiment.

Dötsch V, Matsuo H, Wagner G.

Details

J Magn Reson B. 1996 Jun;111(3):310-3.

Editing for amino-acid type in CBCACONH experiments based on the 13C beta-13C gamma coupling.

Dötsch V, Wagner G.

Details

J Magn Reson B. 1996 Mar;110(3):304-8.

Selective identification of threonine, valine, and isoleucine sequential connectivities with a TVI-CBCACONH experiment.

Dötsch V, Oswald RE, Wagner G.

Details

J Magn Reson B. 1996 Jan;110(1):107-11.

Amino-acid-type-selective triple-resonance experiments.

Dötsch V, Oswald RE, Wagner G.

Details

Biochemistry. 1995 Nov 21;34(46):15057-67.

Exploring the role of the solvent in the denaturation of a protein: a molecular dynamics study of the DNA binding domain of the 434 repressor.

Schiffer CA, Dötsch V, Wüthrich K, van Gunsteren WF.

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FEBS Lett. 1995 Sep 25;372(2-3):288-90.

Salt-stabilized globular protein structure in 7 M aqueous urea solution.

Dötsch V, Wider G, Siegal G, Wüthrich K.

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J Magn Reson B. 1995 Sep;108(3):285-8.

Water suppression by coherence selection with absorptive lineshape without loss in sensitivity.

Dötsch V, Oswald RE, Wagner G.

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FEBS Lett. 1995 Jun 5;366(1):6-10.

Interaction of urea with an unfolded protein. The DNA-binding domain of the 434-repressor.

Dötsch V, Wider G, Siegal G, Wüthrich K.

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