2026
P. Münick, D. Balourdas, J.S. Funk, B. Yüksel, D. Mavridi, J. Heftel, B. Dreier, J.V. Schaefer, B. Schäfer, S. Knapp, T. Telatar, B. Akgül, A. Plückthun, T. Stiewe, A.C. Joerger, & V. Dötsch, DARPins as pan-reactivators of temperature-sensitive p53 cancer mutants, Proc. Natl. Acad. Sci. U.S.A. 123 (18) doi: 10.1073/pnas.2531747123
Dağ Ç, Lambert M, Kazar AE, Kahraman K, Göcenler O, Lee W, Tozkoparan Ceylan CD, Löhr F, Shim JG, Haas AL, Dötsch V, Ziarek J, Elgin ES Dynamic Hotspots in the Uba7 Ubiquitin-Fold Domain Direct UbcH8 Recognition. Biochemistry. 2026 Mar 17, 65(6):678-692. doi: 10.1021/acs.biochem.5c00807
Lenz C, Elson L, Dopfer J, Farges F, Krämer A, Löhr F, Müller S, Guéret SM, Waldmann H, Dötsch V, Saxena K, Knapp S. Overcoming Ligand Discovery Challenges: Developing Peptide-Based Tracers for SPSB2. ACS Chem Biol. 2026 Feb 20, 21(2):274-283. doi: 10.1021/acschembio.5c00702
2025
Merino F, Kock Z, Ermel U, Dahlhaus P, Grimm A, Seybert A, Kubicek J, Frangakis AS, Dotsch V, Hilger D, Bernhard F. Cryo-EM structure of a cell-free synthesized full-length human β1-adrenergic receptor in complex with Gs. Structure 2025, 33(11):1867-1877
Brdar A, Osterburg C, Munick P, Machel AC, Rathore R, Osterburg S, Yuksel B, Schaefer B, Desch K, Langer JD, Dikic I, Dotsch V. p53 isoforms have a high aggregation propensity, interact with chaperones and lack binding to p53 interaction partners. Elife 2025, 13: RP103537.
Sabbarini IM, Reif D, Park K, McQuown AJ, Nelliat AR, Trejtnar C, Dotsch V, Shakhnovich EI, Murray AW, Denic V. A ribosome-associating chaperone mediates GTP-driven vectorial folding of nascent eEF1A. Nat Commun 2025, 16(1): 1277.
Munick P, Strubel A, Balourdas DI, Funk JS, Mernberger M, Osterburg C, Dreier B, Schaefer JV, Tuppi M, Yuksel B, Schafer B, Knapp S, Pluckthun A, Stiewe T, Joerger AC, Dotsch V. DARPin-induced reactivation of p53 in HPV-positive cells. Nat Struct Mol Biol 2025.
Lotz R, Osterburg C, Chaikuad A, Weber S, Akutsu M, Machel AC, Beyer U, Gebel J, Lohr F, Knapp S, Dobbelstein M, Lu X, Dotsch V. Alternative splicing in the DBD linker region of p63 modulates binding to DNA and iASPP in vitro. Cell Death Dis 2025, 16(1): 4.
Gutfreund N, Tuppi M, Schafer B, Klinger FG, Kirchhof A, Hofmann A, Dikic I, Dotsch V. Mechanisms of chemotherapy-induced oocyte death through activation of TAp63alpha. Reproduction 2025, 169(5).
2024
Waltho A, Popp O, Lenz C, Pluska L, Lambert M, Dotsch V, Mertins P, Sommer T. K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors. Life Sci Alliance 2024, 7(8).
Munick P, Zielinski J, Strubel A, Gutfreund N, Dreier B, Schaefer JV, Schafer B, Gebel J, Osterburg C, Chaikuad A, Knapp S, Pluckthun A, Dotsch V. DARPins as a novel tool to detect and degrade p73. Cell Death Dis 2024, 15(12): 909.
Lotz R, Osterburg C, Schafer B, Lu X, Dotsch V. Cardiocutaneous syndrome is caused by aggregation of iASPP mutants. Cell Death Discov 2024, 10(1): 497.
Li D, Kok CYL, Wang C, Ray D, Osterburg S, Dotsch V, Ghosh S, Sabapathy K. Dichotomous transactivation domains contribute to growth inhibitory and promotion functions of TAp73. Proc Natl Acad Sci U S A 2024, 121(21): e2318591121.
Kock Z, Schnelle K, Persechino M, Umbach S, Schihada H, Januliene D, Parey K, Pockes S, Kolb P, Dotsch V, Moller A, Hilger D, Bernhard F. Cryo-EM structure of cell-free synthesized human histamine 2 receptor/G(s) complex in nanodisc environment. Nat Commun 2024, 15(1): 1831.
Kahraman K, Robson SA, Gocenler O, Yenici CM, Tozkoparan Ceylan CD, Klein JM, Dotsch V, Elgin ES, Haas AL, Ziarek JJ, Dag C. Characterizing the Monomer-Dimer Equilibrium of UbcH8/Ube2L6: A Combined SAXS and NMR Study. ACS Omega 2024, 9(38): 39564-39572.
Andreotti AH, Dotsch V. Allosteric regulation of kinase activity. Elife 2024, 13.
2023
Wolf K, Kosinski J, Gibson TJ, Wesch N, Dotsch V, Genuardi M, Cordisco EL, Zeuzem S, Brieger A, Plotz G. A conserved motif in the disordered linker of human MLH1 is vital for DNA mismatch repair and its function is diminished by a cancer family mutation. Nucleic Acids Res 2023, 51(12): 6307-6320.
Strubel A, Munick P, Hartmann O, Chaikuad A, Dreier B, Schaefer JV, Gebel J, Osterburg C, Tuppi M, Schafer B, Buck V, Rosenfeldt M, Knapp S, Pluckthun A, Diefenbacher ME, Dotsch V. DARPins detect the formation of hetero-tetramers of p63 and p73 in epithelial tissues and in squamous cell carcinoma. Cell Death Dis 2023, 14(10): 674.
Smirnov A, Magri A, Lotz R, Han X, Yin C, Harris M, Osterburg C, Dotsch V, McKeating JA, Lu X. ASPP2 binds to hepatitis C virus NS5A protein via an SH3 domain/PxxP motif-mediated interaction and potentiates infection. J Gen Virol 2023, 104(9).
Raab M, Matthess Y, Raab CA, Gutfreund N, Dotsch V, Becker S, Sanhaji M, Strebhardt K. Correction: A dimerization-dependent mechanism regulates enzymatic activation and nuclear entry of PLK1. Oncogene 2023, 42(34): 2590.
Purder PL, Meyners C, Sugiarto WO, Kolos J, Lohr F, Gebel J, Nehls T, Dotsch V, Lermyte F, Hausch F. Deconstructing Protein Binding of Sulfonamides and Sulfonamide Analogues. JACS Au 2023, 3(9): 2478-2486.
Osterburg C, Ferniani M, Antonini D, Frombach AS, D’Auria L, Osterburg S, Lotz R, Lohr F, Kehrloesser S, Zhou H, Missero C, Dotsch V. Disease-related p63 DBD mutations impair DNA binding by distinct mechanisms and varying degree. Cell Death Dis 2023, 14(4): 274.
Mezhyrova J, Martin J, Bornsen C, Dotsch V, Frangakis AS, Morgner N, Bernhard F. In vitro characterization of the phage lysis protein MS2-L. Microbiome Res Rep 2023, 2(4): 28.
Mende H, Khatri A, Lange C, Poveda-Cuevas SA, Tascher G, Covarrubias-Pinto A, Lohr F, Koschade SE, Dikic I, Munch C, Bremm A, Brunetti L, Brandts CH, Uckelmann H, Dotsch V, Rogov VV, Bhaskara RM, Muller S. An atypical GABARAP binding module drives the pro-autophagic potential of the AML-associated NPM1c variant. Cell Rep 2023, 42(12): 113484.
Levin R, Lohr F, Karakoc B, Lichtenecker R, Dotsch V, Bernhard F. E. coli “Stablelabel” S30 lysate for optimized cell-free NMR sample preparation. J Biomol NMR 2023, 77(4): 131-147.
Lambert M, Gebel J, Trejtnar C, Wesch N, Bozkurt S, Adrian-Allgood M, Lohr F, Munch C, Dotsch V. Fuzzy interactions between the auto-phosphorylated C-terminus and the kinase domain of CK1delta inhibits activation of TAp63alpha. Sci Rep 2023, 13(1): 16423.
Kazemi S, Lopata A, Kniss A, Pluska L, Guntert P, Sommer T, Prisner TF, Collauto A, Dotsch V. Efficient determination of the accessible conformation space of multi-domain complexes based on EPR PELDOR data. J Biomol NMR 2023, 77(5-6): 261-269.
Gonzalez A, Covarrubias-Pinto A, Bhaskara RM, Glogger M, Kuncha SK, Xavier A, Seemann E, Misra M, Hoffmann ME, Brauning B, Balakrishnan A, Qualmann B, Dotsch V, Schulman BA, Kessels MM, Hubner CA, Heilemann M, Hummer G, Dikic I. Ubiquitination regulates ER-phagy and remodelling of endoplasmic reticulum. Nature 2023, 618(7964): 394-401.
Berkane R, Ho-Xuan H, Glogger M, Sanz-Martinez P, Brunello L, Glaesner T, Kuncha SK, Holzhuter K, Cano-Franco S, Buonomo V, Cabrerizo-Poveda P, Balakrishnan A, Tascher G, Husnjak K, Juretschke T, Misra M, Gonzalez A, Dotsch V, Grumati P, Heilemann M, Stolz A. The function of ER-phagy receptors is regulated through phosphorylation-dependent ubiquitination pathways. Nat Commun 2023, 14(1): 8364.
2022
Umbach S, Levin R, Neumann S, Steinmetzer T, Dotsch V, Bernhard F. Transfer mechanism of cell-free synthesized membrane proteins into mammalian cells. Front Bioeng Biotechnol 2022, 10: 906295.
Umbach S, Dotsch V, Bernhard F. Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies. Methods Mol Biol 2022, 2507: 405-424.
Tucker EJ, Gutfreund N, Belaud-Rotureau MA, Gilot D, Brun T, Kline BL, Bell KM, Domin-Bernhard M, Theard C, Touraine P, Robevska G, van van den Bergen J, Ayers KL, Sinclair AH, Dotsch V, Jaillard S. Dominant TP63 missense variants lead to constitutive activation and premature ovarian insufficiency. Hum Mutat 2022, 43(10): 1443-1453.
Strubel A, Munick P, Chaikuad A, Dreier B, Schaefer J, Gebel J, Osterburg C, Tuppi M, Schafer B, Knapp S, Pluckthun A, Dotsch V. Designed Ankyrin Repeat Proteins as a tool box for analyzing p63. Cell Death Differ 2022, 29(12): 2445-2458.
Raab M, Matthess Y, Raab CA, Gutfreund N, Dotsch V, Becker S, Sanhaji M, Strebhardt K. A dimerization-dependent mechanism regulates enzymatic activation and nuclear entry of PLK1. Oncogene 2022, 41(3): 372-386.
Osterburg C, Dotsch V. Structural diversity of p63 and p73 isoforms. Cell Death Differ 2022, 29(5): 921-937.
Mezhyrova J, Mors K, Glaubitz C, Dotsch V, Bernhard F. Applications of Cell-Free Synthesized Membrane Protein Precipitates. Methods Mol Biol 2022, 2406: 245-266.
Levin R, Kock Z, Martin J, Zangl R, Gewering T, Schuler L, Moeller A, Dotsch V, Morgner N, Bernhard F. Cotranslational assembly of membrane protein/nanoparticles in cell-free systems. Biochim Biophys Acta Biomembr 2022, 1864(11): 184017.
Kock Z, Ermel U, Martin J, Morgner N, Frangakis AS, Dotsch V, Hilger D, Bernhard F. Biochemical Characterization of Cell-free Synthesized Human beta(1) Adrenergic Receptor Cotranslationally Inserted into Nanodiscs. J Mol Biol 2022, 434(16): 167687.
Gruijs da Silva LA, Simonetti F, Hutten S, Riemenschneider H, Sternburg EL, Pietrek LM, Gebel J, Dotsch V, Edbauer D, Hummer G, Stelzl LS, Dormann D. Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation. EMBO J 2022, 41(8): e108443.
Fester N, Zielonka E, Goldmann J, Frombach AS, Muller-Kuller U, Gutfreund N, Riegel K, Smits JGA, Schleiff E, Rajalingam K, Zhou H, Simm S, Dotsch V. Enhanced pro-apoptosis gene signature following the activation of TAp63alpha in oocytes upon gamma irradiation. Cell Death Dis 2022, 13(3): 204.
Cullati SN, Chaikuad A, Chen JS, Gebel J, Tesmer L, Zhubi R, Navarrete-Perea J, Guillen RX, Gygi SP, Hummer G, Dotsch V, Knapp S, Gould KL. Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes. Mol Cell 2022, 82(11): 2006-2020 e2008.
Al Moussawi K, Chung K, Carroll TM, Osterburg C, Smirnov A, Lotz R, Miller P, Dedeic Z, Zhong S, Oti M, Kouwenhoven EN, Asher R, Goldin R, Tellier M, Murphy S, Zhou H, Dotsch V, Lu X. Mutant Ras and inflammation-driven skin tumorigenesis is suppressed via a JNK-iASPP-AP1 axis. Cell Rep 2022, 41(3): 111503.
2021
Wesch N, Lohr F, Rogova N, Dotsch V, Rogov VV. A Concerted Action of UBA5 C-Terminal Unstructured Regions Is Important for Transfer of Activated UFM1 to UFC1. Int J Mol Sci 2021, 22(14).
Pluska L, Jarosch E, Zauber H, Kniss A, Waltho A, Bagola K, von Delbruck M, Lohr F, Schulman BA, Selbach M, Dotsch V, Sommer T. The UBA domain of conjugating enzyme Ubc1/Ube2K facilitates assembly of K48/K63-branched ubiquitin chains. EMBO J 2021, 40(6): e106094.
Osterburg C, Osterburg S, Zhou H, Missero C, Dotsch V. Isoform-Specific Roles of Mutant p63 in Human Diseases. Cancers (Basel) 2021, 13(3).
Mezhyrova J, Martin J, Peetz O, Dotsch V, Morgner N, Ma Y, Bernhard F. Membrane insertion mechanism and molecular assembly of the bacteriophage lysis toxin PhiX174-E. FEBS J 2021, 288(10): 3300-3316.
Lena AM, Rossi V, Osterburg S, Smirnov A, Osterburg C, Tuppi M, Cappello A, Amelio I, Dotsch V, De Felici M, Klinger FG, Annicchiarico-Petruzzelli M, Valensise H, Melino G, Candi E. The p63 C-terminus is essential for murine oocyte integrity. Nat Commun 2021, 12(1): 383.
Kock Z, Dotsch V, Bernhard F. Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples. Methods Mol Biol 2021, 2268: 97-117.
Hartmann M, Huber J, Kramer JS, Heering J, Pietsch L, Stark H, Odadzic D, Bischoff I, Furst R, Schroder M, Akutsu M, Chaikuad A, Dotsch V, Knapp S, Biondi RM, Rogov VV, Proschak E. Demonstrating Ligandability of the LC3A and LC3B Adapter Interface. J Med Chem 2021, 64(7): 3720-3746.
Dolle A, Adhikari B, Kramer A, Weckesser J, Berner N, Berger LM, Diebold M, Szewczyk MM, Barsyte-Lovejoy D, Arrowsmith CH, Gebel J, Lohr F, Dotsch V, Eilers M, Heinzlmeir S, Kuster B, Sotriffer C, Wolf E, Knapp S. Design, Synthesis, and Evaluation of WD-Repeat-Containing Protein 5 (WDR5) Degraders. J Med Chem 2021, 64(15): 10682-10710.
Di Rita A, Angelini DF, Maiorino T, Caputo V, Cascella R, Kumar M, Tiberti M, Lambrughi M, Wesch N, Lohr F, Dotsch V, Carinci M, D’Acunzo P, Chiurchiu V, Papaleo E, Rogov VV, Giardina E, Battistini L, Strappazzon F. Characterization of a natural variant of human NDP52 and its functional consequences on mitophagy. Cell Death Differ 2021, 28(8): 2499-2516.
Candi E, Melino G, Toth A, Dotsch V. Mechanisms of quality control differ in male and female germ cells. Cell Death Differ 2021, 28(7): 2300-2302.
Broft P, Dzatko S, Krafcikova M, Wacker A, Hansel-Hertsch R, Dotsch V, Trantirek L, Schwalbe H. In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells. Angew Chem Int Ed Engl 2021, 60(2): 865-872.
2020
Timofeev O, Koch L, Niederau C, Tscherne A, Schneikert J, Klimovich M, Elmshauser S, Zeitlinger M, Mernberger M, Nist A, Osterburg C, Dotsch V, Hrabe de Angelis M, Stiewe T, German Mouse Clinic C. Phosphorylation Control of p53 DNA-Binding Cooperativity Balances Tumorigenesis and Aging. Cancer Res 2020, 80(23): 5231-5244.
Shin D, Mukherjee R, Liu Y, Gonzalez A, Bonn F, Liu Y, Rogov VV, Heinz M, Stolz A, Hummer G, Dotsch V, Luo ZQ, Bhogaraju S, Dikic I. Regulation of Phosphoribosyl-Linked Serine Ubiquitination by Deubiquitinases DupA and DupB. Mol Cell 2020, 77(1): 164-179 e166.
Mader J, Huber J, Bonn F, Dotsch V, Rogov VV, Bremm A. Oxygen-dependent asparagine hydroxylation of the ubiquitin-associated (UBA) domain in Cezanne regulates ubiquitin binding. J Biol Chem 2020, 295(8): 2160-2174.
Maculins T, Garcia-Pardo J, Skenderovic A, Gebel J, Putyrski M, Vorobyov A, Busse P, Varga G, Kuzikov M, Zaliani A, Rahighi S, Schaeffer V, Parnham MJ, Sidhu SS, Ernst A, Dotsch V, Akutsu M, Dikic I. Discovery of Protein-Protein Interaction Inhibitors by Integrating Protein Engineering and Chemical Screening Platforms. Cell Chem Biol 2020, 27(11): 1441-1451 e1447.
Lopata A, Kniss A, Lohr F, Rogov VV, Dotsch V. Ubiquitination in the ERAD Process. Int J Mol Sci 2020, 21(15).
Levin R, Koeck Z, Dotsch V, Bernhard F. Co-translational Insertion of Membrane Proteins into Preformed Nanodiscs. J Vis Exp 2020(165).
Huber J, Obata M, Gruber J, Akutsu M, Lohr F, Rogova N, Guntert P, Dikic I, Kirkin V, Komatsu M, Dotsch V, Rogov VV. An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5. Autophagy 2020, 16(2): 256-270.
Gebel J, Tuppi M, Sanger N, Schumacher B, Dotsch V. DNA Damaged Induced Cell Death in Oocytes. Molecules 2020, 25(23).
Gebel J, Tuppi M, Chaikuad A, Hotte K, Schroder M, Schulz L, Lohr F, Gutfreund N, Finke F, Henrich E, Mezhyrova J, Lehnert R, Pampaloni F, Hummer G, Stelzer EHK, Knapp S, Dotsch V. p63 uses a switch-like mechanism to set the threshold for induction of apoptosis. Nat Chem Biol 2020, 16(10): 1078-1086.
Duchatelet S, Russo C, Osterburg C, Mallet S, Bole-Feysot C, Nitschke P, Richard MA, Dotsch V, Missero C, Nassif A, Hovnanian A. A TP63 Mutation Causes Prominent Alopecia with Mild Ectodermal Dysplasia. J Invest Dermatol 2020, 140(5): 1103-1106 e1104.
2010-2019
Pitzius S, Osterburg C, Gebel J, Tascher G, Schafer B, Zhou H, Munch C, Dotsch V. TA*p63 and GTAp63 achieve tighter transcriptional regulation in quality control by converting an inhibitory element into an additional transactivation domain. Cell Death Dis 2019, 10(10): 686.
Peetz O, Hellwig N, Henrich E, Mezhyrova J, Dotsch V, Bernhard F, Morgner N. LILBID and nESI: Different Native Mass Spectrometry Techniques as Tools in Structural Biology. J Am Soc Mass Spectrom 2019, 30(1): 181-191.
Lohr F, Gebel J, Henrich E, Hein C, Dotsch V. Towards complete polypeptide backbone NH assignment via combinatorial labeling. J Magn Reson 2019, 302: 50-63.
Khandelwal KD, van den Boogaard MH, Mehrem SL, Gebel J, Fagerberg C, van Beusekom E, van Binsbergen E, Topaloglu O, Steehouwer M, Gilissen C, Ishorst N, van Rooij I, Roeleveld N, Christensen K, Schoenaers J, Berge S, Murray JC, Hens G, Devriendt K, Ludwig KU, Mangold E, Hoischen A, Zhou H, Dotsch V, Carels CEL, van Bokhoven H. Deletions and loss-of-function variants in TP63 associated with orofacial clefting. Eur J Hum Genet 2019, 27(7): 1101-1112.
Keller T, Gorboulev V, Mueller TD, Dotsch V, Bernhard F, Koepsell H. Rat Organic Cation Transporter 1 Contains Three Binding Sites for Substrate 1-Methyl-4-phenylpyridinium per Monomer. Mol Pharmacol 2019, 95(2): 169-182.
Holdgaard SG, Cianfanelli V, Pupo E, Lambrughi M, Lubas M, Nielsen JC, Eibes S, Maiani E, Harder LM, Wesch N, Foged MM, Maeda K, Nazio F, de la Ballina LR, Dotsch V, Brech A, Frankel LB, Jaattela M, Locatelli F, Barisic M, Andersen JS, Bekker-Jensen S, Lund AH, Rogov VV, Papaleo E, Lanzetti L, De Zio D, Cecconi F. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 2019, 10(1): 4176.
Henrich E, Lohr F, Mezhyrova J, Laguerre A, Bernhard F, Dotsch V. Synthetic Biology-Based Solution NMR Studies on Membrane Proteins in Lipid Environments. Methods Enzymol 2019, 614: 143-185.
Frank T, Tuppi M, Hugle M, Dotsch V, van Wijk SJL, Fulda S. Cell cycle arrest in mitosis promotes interferon-induced necroptosis. Cell Death Differ 2019, 26(10): 2046-2060.
Tuppi M, Kehrloesser S, Coutandin DW, Rossi V, Luh LM, Strubel A, Hotte K, Hoffmeister M, Schafer B, De Oliveira T, Greten F, Stelzer EHK, Knapp S, De Felici M, Behrends C, Klinger FG, Dotsch V. Oocyte DNA damage quality control requires consecutive interplay of CHK2 and CK1 to activate p63. Nat Struct Mol Biol 2018, 25(3): 261-269.
Russo C, Osterburg C, Sirico A, Antonini D, Ambrosio R, Wurz JM, Rinnenthal J, Ferniani M, Kehrloesser S, Schafer B, Guntert P, Sinha S, Dotsch V, Missero C. Protein aggregation of the p63 transcription factor underlies severe skin fragility in AEC syndrome. Proc Natl Acad Sci U S A 2018, 115(5): E906-E915.
Rues RB, Dong F, Dotsch V, Bernhard F. Systematic optimization of cell-free synthesized human endothelin B receptor folding. Methods 2018, 147: 73-83.
Rogov VV, Stolz A, Ravichandran AC, Rios-Szwed DO, Suzuki H, Kniss A, Lohr F, Wakatsuki S, Dotsch V, Dikic I, Dobson RC, McEwan DG. Structural and functional analysis of the GABARAP interaction motif (GIM). EMBO Rep 2018, 19(12).
Nemajerova A, Amelio I, Gebel J, Dotsch V, Melino G, Moll UM. Non-oncogenic roles of TAp73: from multiciliogenesis to metabolism. Cell Death Differ 2018, 25(1): 144-153.
Muller S, Ackloo S, Arrowsmith CH, Bauser M, Baryza JL, Blagg J, Bottcher J, Bountra C, Brown PJ, Bunnage ME, Carter AJ, Damerell D, Dotsch V, Drewry DH, Edwards AM, Edwards J, Elkins JM, Fischer C, Frye SV, Gollner A, Grimshaw CE, A IJ, Hanke T, Hartung IV, Hitchcock S, Howe T, Hughes TV, Laufer S, Li VM, Liras S, Marsden BD, Matsui H, Mathias J, O’Hagan RC, Owen DR, Pande V, Rauh D, Rosenberg SH, Roth BL, Schneider NS, Scholten C, Singh Saikatendu K, Simeonov A, Takizawa M, Tse C, Thompson PR, Treiber DK, Viana AY, Wells CI, Willson TM, Zuercher WJ, Knapp S, Mueller-Fahrnow A. Donated chemical probes for open science. Elife 2018, 7.
Lazarova M, Lohr F, Rues RB, Kleebach R, Dotsch V, Bernhard F. Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins. ACS Chem Biol 2018, 13(8): 2170-2178.
Krauskopf K, Gebel J, Kazemi S, Tuppi M, Lohr F, Schafer B, Koch J, Guntert P, Dotsch V, Kehrloesser S. Regulation of the Activity in the p53 Family Depends on the Organization of the Transactivation Domain. Structure 2018, 26(8): 1091-1100 e1094.
Kniss A, Schuetz D, Kazemi S, Pluska L, Spindler PE, Rogov VV, Husnjak K, Dikic I, Guntert P, Sommer T, Prisner TF, Dotsch V. Chain Assembly and Disassembly Processes Differently Affect the Conformational Space of Ubiquitin Chains. Structure 2018, 26(2): 249-258 e244.
Kniss A, Kazemi S, Lohr F, Berger M, Rogov VV, Guntert P, Sommer T, Jarosch E, Dotsch V. Structural investigation of glycan recognition by the ERAD quality control lectin Yos9. J Biomol NMR 2018, 72(1-2): 1-10.
Kehrloesser S, Tuppi M, Dotsch V. CHK2 sets the stage for CK1 in oocyte quality control. Cell Death Differ 2018, 25(6): 1007-1009.
Hoffmann B, Lohr F, Laguerre A, Bernhard F, Dotsch V. Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis. Prog Nucl Magn Reson Spectrosc 2018, 105: 1-22.
Henrich E, Lohr F, Pawlik G, Peetz O, Dotsch V, Morgner N, de Kroon AI, Bernhard F. Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism. Biochemistry 2018, 57(40): 5780-5784.
Foshag D, Henrich E, Hiller E, Schafer M, Kerger C, Burger-Kentischer A, Diaz-Moreno I, Garcia-Maurino SM, Dotsch V, Rupp S, Bernhard F. The E. coli S30 lysate proteome: A prototype for cell-free protein production. N Biotechnol 2018, 40(Pt B): 245-260.
Dong F, Rues RB, Kazemi S, Dotsch V, Bernhard F. Molecular Determinants for Ligand Selectivity of the Cell-Free Synthesized Human Endothelin B Receptor. J Mol Biol 2018, 430(24): 5105-5119.
Di Rita A, Peschiaroli A, P DA, Strobbe D, Hu Z, Gruber J, Nygaard M, Lambrughi M, Melino G, Papaleo E, Dengjel J, El Alaoui S, Campanella M, Dotsch V, Rogov VV, Strappazzon F, Cecconi F. HUWE1 E3 ligase promotes PINK1/PARKIN-independent mitophagy by regulating AMBRA1 activation via IKKalpha. Nat Commun 2018, 9(1): 3755.
Bock C, Lohr F, Tumulka F, Reichel K, Wurz J, Hummer G, Schafer L, Tampe R, Joseph B, Bernhard F, Dotsch V, Abele R. Structural and functional insights into the interaction and targeting hub TMD0 of the polypeptide transporter TAPL. Sci Rep 2018, 8(1): 15662.
Wiechmann S, Gartner A, Kniss A, Stengl A, Behrends C, Rogov VV, Rodriguez MS, Dotsch V, Muller S, Ernst A. Site-specific inhibition of the small ubiquitin-like modifier (SUMO)-conjugating enzyme Ubc9 selectively impairs SUMO chain formation. J Biol Chem 2017, 292(37): 15340-15351.
Waberer L, Henrich E, Peetz O, Morgner N, Dotsch V, Bernhard F, Volknandt W. The synaptic vesicle protein SV31 assembles into a dimer and transports Zn(2). J Neurochem 2017, 140(2): 280-293.
Stolz A, Putyrski M, Kutle I, Huber J, Wang C, Major V, Sidhu SS, Youle RJ, Rogov VV, Dotsch V, Ernst A, Dikic I. Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins. EMBO J 2017, 36(4): 549-564.
Rogov VV, Suzuki H, Marinkovic M, Lang V, Kato R, Kawasaki M, Buljubasic M, Sprung M, Rogova N, Wakatsuki S, Hamacher-Brady A, Dotsch V, Dikic I, Brady NR, Novak I. Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins. Sci Rep 2017, 7(1): 1131.
Rogov VV, Stolz A, Ravichandran AC, Rios-Szwed DO, Suzuki H, Kniss A, Lohr F, Wakatsuki S, Dotsch V, Dikic I, Dobson RC, McEwan DG. Structural and functional analysis of the GABARAP interaction motif (GIM). EMBO Rep 2017, 18(8): 1382-1396.
Reckel S, Gehin C, Tardivon D, Georgeon S, Kukenshoner T, Lohr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Guntert P, Dotsch V, Koide S, Gavin AC, Hantschel O. Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase. Nat Commun 2017, 8(1): 2101.
Peetz O, Henrich E, Laguerre A, Lohr F, Hein C, Dotsch V, Bernhard F, Morgner N. Insights into Cotranslational Membrane Protein Insertion by Combined LILBID-Mass Spectrometry and NMR Spectroscopy. Anal Chem 2017, 89(22): 12314-12318.
Johansen T, Birgisdottir AB, Huber J, Kniss A, Dotsch V, Kirkin V, Rogov VV. Methods for Studying Interactions Between Atg8/LC3/GABARAP and LIR-Containing Proteins. Methods Enzymol 2017, 587: 143-169.
Imre G, Berthelet J, Heering J, Kehrloesser S, Melzer IM, Lee BI, Thiede B, Dotsch V, Rajalingam K. Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2. EMBO Rep 2017, 18(5): 733-744.
Henrich E, Sormann J, Eberhardt P, Peetz O, Mezhyrova J, Morgner N, Fendler K, Dotsch V, Wachtveitl J, Bernhard F, Bamann C. From Gene to Function: Cell-Free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs. Biophys J 2017, 113(6): 1331-1341.
Henrich E, Peetz O, Hein C, Laguerre A, Hoffmann B, Hoffmann J, Dotsch V, Bernhard F, Morgner N. Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology. Elife 2017, 6.
Hein C, Lohr F, Schwarz D, Dotsch V. Acceleration of protein backbone NMR assignment by combinatorial labeling: Application to a small molecule binding study. Biopolymers 2017, 107(5).
Gebel J, Tuppi M, Krauskopf K, Coutandin D, Pitzius S, Kehrloesser S, Osterburg C, Dotsch V. Control mechanisms in germ cells mediated by p53 family proteins. J Cell Sci 2017.
Chillemi G, Kehrloesser S, Bernassola F, Desideri A, Dotsch V, Levine AJ, Melino G. Structural Evolution and Dynamics of the p53 Proteins. Cold Spring Harb Perspect Med 2017, 7(4).
von Delbruck M, Kniss A, Rogov VV, Pluska L, Bagola K, Lohr F, Guntert P, Sommer T, Dotsch V. The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation. Mol Cell 2016, 62(6): 918-928.
Rues RB, Dotsch V, Bernhard F. Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments. Biochim Biophys Acta 2016, 1858(6): 1306-1316.
Laguerre A, Lohr F, Henrich E, Hoffmann B, Abdul-Manan N, Connolly PJ, Perozo E, Moore JM, Bernhard F, Dotsch V. From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins. Structure 2016, 24(10): 1830-1841.
Kehrloesser S, Osterburg C, Tuppi M, Schafer B, Vousden KH, Dotsch V. Intrinsic aggregation propensity of the p63 and p73 TI domains correlates with p53R175H interaction and suggests further significance of aggregation events in the p53 family. Cell Death Differ 2016, 23(12): 1952-1960.
Henrich E, Ma Y, Engels I, Munch D, Otten C, Schneider T, Henrichfreise B, Sahl HG, Dotsch V, Bernhard F. Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway. J Biol Chem 2016, 291(5): 2535-2546.
Heering J, Jonker HR, Lohr F, Schwalbe H, Dotsch V. Structural investigations of the p53/p73 homologs from the tunicate species Ciona intestinalis reveal the sequence requirements for the formation of a tetramerization domain. Protein Sci 2016, 25(2): 410-422.
Habisov S, Huber J, Ichimura Y, Akutsu M, Rogova N, Loehr F, McEwan DG, Johansen T, Dikic I, Doetsch V, Komatsu M, Rogov VV, Kirkin V. Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. J Biol Chem 2016, 291(17): 9025-9041.
Gebel J, Luh LM, Coutandin D, Osterburg C, Lohr F, Schafer B, Frombach AS, Sumyk M, Buchner L, Krojer T, Salah E, Mathea S, Guntert P, Knapp S, Dotsch V. Mechanism of TAp73 inhibition by DeltaNp63 and structural basis of p63/p73 hetero-tetramerization. Cell Death Differ 2016, 23(12): 1930-1940.
Focke PJ, Hein C, Hoffmann B, Matulef K, Bernhard F, Dotsch V, Valiyaveetil FI. Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression. Biochemistry 2016, 55(30): 4212-4219.
Coutandin D, Osterburg C, Srivastav RK, Sumyk M, Kehrloesser S, Gebel J, Tuppi M, Hannewald J, Schafer B, Salah E, Mathea S, Muller-Kuller U, Doutch J, Grez M, Knapp S, Dotsch V. Quality control in oocytes by p63 is based on a spring-loaded activation mechanism on the molecular and cellular level. Elife 2016, 5.
Stindt MH, Muller PA, Ludwig RL, Kehrloesser S, Dotsch V, Vousden KH. Functional interplay between MDM2, p63/p73 and mutant p53. Oncogene 2015, 34(33): 4300-4310.
Stadel D, Millarte V, Tillmann KD, Huber J, Tamin-Yecheskel BC, Akutsu M, Demishtein A, Ben-Zeev B, Anikster Y, Perez F, Dotsch V, Elazar Z, Rogov V, Farhan H, Behrends C. TECPR2 Cooperates with LC3C to Regulate COPII-Dependent ER Export. Mol Cell 2015, 60(1): 89-104.
Schwamb B, Pick R, Fernandez SB, Volp K, Heering J, Dotsch V, Bosser S, Jung J, Beinoraviciute-Kellner R, Wesely J, Zornig I, Hammerschmidt M, Nowak M, Penzel R, Zatloukal K, Joos S, Rieker RJ, Agaimy A, Soder S, Reid-Lombardo KM, Kendrick ML, Bardsley MR, Hayashi Y, Asuzu DT, Syed SA, Ordog T, Zornig M. FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. Int J Cancer 2015, 137(6): 1318-1329.
Orban E, Proverbio D, Haberstock S, Dotsch V, Bernhard F. Cell-free expression of G-protein-coupled receptors. Methods Mol Biol 2015, 1261: 171-195.
Merk H, Rues RB, Gless C, Beyer K, Dong F, Dotsch V, Gerrits M, Bernhard F. Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing. Biol Chem 2015, 396(9-10): 1097-1107.
Mehler M, Eckert CE, Busche A, Kulhei J, Michaelis J, Becker-Baldus J, Wachtveitl J, Dotsch V, Glaubitz C. Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing. J Biol Chem 2015, 290(46): 27712-27722.
Lohr F, Tumulka F, Bock C, Abele R, Dotsch V. An extended combinatorial 15N, 13Calpha, and 13C’ labeling approach to protein backbone resonance assignment. J Biomol NMR 2015, 62(3): 263-279.
LaGuerre A, Lohr F, Bernhard F, Dotsch V. Labeling of membrane proteins by cell-free expression. Methods Enzymol 2015, 565: 367-388.
Klingler FM, Moser D, Buttner D, Wichelhaus TA, Lohr F, Dotsch V, Proschak E. Probing metallo-beta-lactamases with molecular fragments identified by consensus docking. Bioorg Med Chem Lett 2015, 25(22): 5243-5246.
Kai L, Orban E, Henrich E, Proverbio D, Dotsch V, Bernhard F. Co-translational stabilization of insoluble proteins in cell-free expression systems. Methods Mol Biol 2015, 1258: 125-143.
Henrich E, Hein C, Dotsch V, Bernhard F. Membrane protein production in Escherichia coli cell-free lysates. FEBS Lett 2015, 589(15): 1713-1722.
Henrich E, Dotsch V, Bernhard F. Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs. Methods Enzymol 2015, 556: 351-369.
Genau HM, Huber J, Baschieri F, Akutsu M, Dotsch V, Farhan H, Rogov V, Behrends C. CUL3-KBTBD6/KBTBD7 ubiquitin ligase cooperates with GABARAP proteins to spatially restrict TIAM1-RAC1 signaling. Mol Cell 2015, 57(6): 995-1010.
Chatterjee D, Kudlinzki D, Linhard V, Saxena K, Schieborr U, Gande SL, Wurm JP, Wohnert J, Abele R, Rogov VV, Dotsch V, Osiewacz HD, Sreeramulu S, Schwalbe H. Structure and Biophysical Characterization of the S-Adenosylmethionine-dependent O-Methyltransferase PaMTH1, a Putative Enzyme Accumulating during Senescence of Podospora anserina. J Biol Chem 2015, 290(26): 16415-16430.
Tufar P, Rahighi S, Kraas FI, Kirchner DK, Lohr F, Henrich E, Kopke J, Dikic I, Guntert P, Marahiel MA, Dotsch V. Crystal structure of a PCP/Sfp complex reveals the structural basis for carrier protein posttranslational modification. Chem Biol 2014, 21(4): 552-562.
Tikole S, Jaravine V, Rogov V, Dotsch V, Guntert P. Peak picking NMR spectral data using non-negative matrix factorization. BMC Bioinformatics 2014, 15: 46.
Schroder M, Hafner AK, Hofmann B, Radmark O, Tumulka F, Abele R, Dotsch V, Steinhilber D. Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase. Biochim Biophys Acta 2014, 1842(10): 1538-1547.
Rues RB, Orban E, Dotsch V, Bernhard F. Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets. Biol Chem 2014, 395(12): 1425-1434.
Roos C, Kai L, Haberstock S, Proverbio D, Ghoshdastider U, Ma Y, Filipek S, Wang X, Dotsch V, Bernhard F. High-level cell-free production of membrane proteins with nanodiscs. Methods Mol Biol 2014, 1118: 109-130.
Rogov V, Dotsch V, Johansen T, Kirkin V. Interactions between autophagy receptors and ubiquitin-like proteins form the molecular basis for selective autophagy. Mol Cell 2014, 53(2): 167-178.
Lohr F, Laguerre A, Bock C, Reckel S, Connolly PJ, Abdul-Manan N, Tumulka F, Abele R, Moore JM, Dotsch V. Time-shared experiments for efficient assignment of triple-selectively labeled proteins. J Magn Reson 2014, 248: 81-95.
Hansel R, Luh LM, Corbeski I, Trantirek L, Dotsch V. In-cell NMR and EPR spectroscopy of biomacromolecules. Angew Chem Int Ed Engl 2014, 53(39): 10300-10314.
Boland C, Li D, Shah STA, Haberstock S, Dotsch V, Bernhard F, Caffrey M. Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination. Cell Mol Life Sci 2014, 71(24): 4895-4910.
Zettler J, Eppmann S, Busche A, Dikovskaya D, Dotsch V, Mootz HD, Sonntag T. SPLICEFINDER – a fast and easy screening method for active protein trans-splicing positions. PLoS One 2013, 8(9): e72925.
Tumulka F, Roos C, Lohr F, Bock C, Bernhard F, Dotsch V, Abele R. Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR. J Biomol NMR 2013, 57(2): 141-154.
Roos C, Kai L, Proverbio D, Ghoshdastider U, Filipek S, Dotsch V, Bernhard F. Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers. Mol Membr Biol 2013, 30(1): 75-89.
Rogov VV, Suzuki H, Fiskin E, Wild P, Kniss A, Rozenknop A, Kato R, Kawasaki M, McEwan DG, Lohr F, Guntert P, Dikic I, Wakatsuki S, Dotsch V. Structural basis for phosphorylation-triggered autophagic clearance of Salmonella. Biochem J 2013, 454(3): 459-466.
Proverbio D, Roos C, Beyermann M, Orban E, Dotsch V, Bernhard F. Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments. Biochim Biophys Acta 2013, 1828(9): 2182-2192.
Mors K, Roos C, Scholz F, Wachtveitl J, Dotsch V, Bernhard F, Glaubitz C. Modified lipid and protein dynamics in nanodiscs. Biochim Biophys Acta 2013, 1828(4): 1222-1229.
Luh LM, Kehrloesser S, Deutsch GB, Gebel J, Coutandin D, Schafer B, Agostini M, Melino G, Dotsch V. Analysis of the oligomeric state and transactivation potential of TAp73alpha. Cell Death Differ 2013, 20(8): 1008-1016.
Luh LM, Hansel R, Lohr F, Kirchner DK, Krauskopf K, Pitzius S, Schafer B, Tufar P, Corbeski I, Guntert P, Dotsch V. Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition. J Am Chem Soc 2013, 135(37): 13796-13803.
Kai L, Dotsch V, Kaldenhoff R, Bernhard F. Artificial environments for the co-translational stabilization of cell-free expressed proteins. PLoS One 2013, 8(2): e56637.
Hein C, Wittinghofer A, Dotsch V. How to switch a master switch. Elife 2013, 2: e01159.
Hansel R, Lohr F, Trantirek L, Dotsch V. High-resolution insight into G-overhang architecture. J Am Chem Soc 2013, 135(7): 2816-2824.
Hansel R, Foldynova-Trantirkova S, Dotsch V, Trantirek L. Investigation of quadruplex structure under physiological conditions using in-cell NMR. Top Curr Chem 2013, 330: 47-65.
Denic V, Dotsch V, Sinning I. Endoplasmic reticulum targeting and insertion of tail-anchored membrane proteins by the GET pathway. Cold Spring Harb Perspect Biol 2013, 5(8): a013334.
Zocher M, Roos C, Wegmann S, Bosshart PD, Dotsch V, Bernhard F, Muller DJ. Single-molecule force spectroscopy from nanodiscs: an assay to quantify folding, stability, and interactions of native membrane proteins. ACS Nano 2012, 6(1): 961-971.
Tucci P, Agostini M, Grespi F, Markert EK, Terrinoni A, Vousden KH, Muller PA, Dotsch V, Kehrloesser S, Sayan BS, Giaccone G, Lowe SW, Takahashi N, Vandenabeele P, Knight RA, Levine AJ, Melino G. Loss of p63 and its microRNA-205 target results in enhanced cell migration and metastasis in prostate cancer. Proc Natl Acad Sci U S A 2012, 109(38): 15312-15317.
Tikole S, Jaravine V, Rogov VV, Rozenknop A, Schmoe K, Lohr F, Dotsch V, Guntert P. Fast automated NMR spectroscopy of short-lived biological samples. Chembiochem 2012, 13(7): 964-967.
Stehle J, Scholz F, Lohr F, Reckel S, Roos C, Blum M, Braun M, Glaubitz C, Dotsch V, Wachtveitl J, Schwalbe H. Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies. J Biomol NMR 2012, 54(4): 401-413.
Schneidman-Duhovny D, Rossi A, Avila-Sakar A, Kim SJ, Velazquez-Muriel J, Strop P, Liang H, Krukenberg KA, Liao M, Kim HM, Sobhanifar S, Dotsch V, Rajpal A, Pons J, Agard DA, Cheng Y, Sali A. A method for integrative structure determination of protein-protein complexes. Bioinformatics 2012, 28(24): 3282-3289.
Roos C, Zocher M, Muller D, Munch D, Schneider T, Sahl HG, Scholz F, Wachtveitl J, Ma Y, Proverbio D, Henrich E, Dotsch V, Bernhard F. Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase. Biochim Biophys Acta 2012, 1818(12): 3098-3106.
Rogov VV, Rozenknop A, Rogova NY, Lohr F, Tikole S, Jaravine V, Guntert P, Dikic I, Dotsch V. A universal expression tag for structural and functional studies of proteins. Chembiochem 2012, 13(7): 959-963.
Reckel S, Lopez JJ, Lohr F, Glaubitz C, Dotsch V. In-cell solid-state NMR as a tool to study proteins in large complexes. Chembiochem 2012, 13(4): 534-537.
Ma Y, Ghoshdastider U, Wang J, Ye W, Dotsch V, Filipek S, Bernhard F, Wang X. Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening. Protein Expr Purif 2012, 86(2): 120-126.
Lohr F, Reckel S, Karbyshev M, Connolly PJ, Abdul-Manan N, Bernhard F, Moore JM, Dotsch V. Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment. J Biomol NMR 2012, 52(3): 197-210.
Kai L, Roos C, Haberstock S, Proverbio D, Ma Y, Junge F, Karbyshev M, Dotsch V, Bernhard F. Systems for the cell-free synthesis of proteins. Methods Mol Biol 2012, 800: 201-225.
Imre G, Heering J, Takeda AN, Husmann M, Thiede B, zu Heringdorf DM, Green DR, van der Goot FG, Sinha B, Dotsch V, Rajalingam K. Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis. EMBO J 2012, 31(11): 2615-2628.
Haberstock S, Roos C, Hoevels Y, Dotsch V, Schnapp G, Pautsch A, Bernhard F. A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems. Protein Expr Purif 2012, 82(2): 308-316.
Gottstein D, Reckel S, Dotsch V, Guntert P. Requirements on paramagnetic relaxation enhancement data for membrane protein structure determination by NMR. Structure 2012, 20(6): 1019-1027.
Busche A, Gottstein D, Hein C, Ripin N, Pader I, Tufar P, Eisman EB, Gu L, Walsh CT, Sherman DH, Lohr F, Guntert P, Dotsch V. Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthase. ACS Chem Biol 2012, 7(2): 378-386.
Wild P, Farhan H, McEwan DG, Wagner S, Rogov VV, Brady NR, Richter B, Korac J, Waidmann O, Choudhary C, Dotsch V, Bumann D, Dikic I. Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science 2011, 333(6039): 228-233.
Stefer S, Reitz S, Wang F, Wild K, Pang YY, Schwarz D, Bomke J, Hein C, Lohr F, Bernhard F, Denic V, Dotsch V, Sinning I. Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. Science 2011, 333(6043): 758-762.
Sripathomsawat W, Tanpaiboon P, Heering J, Dotsch V, Hennekam RC, Kantaputra P. Phenotypic analysis of Arg227 mutations of TP63 with emphasis on dental phenotype and micturition difficulties in EEC syndrome. Am J Med Genet A 2011, 155A(1): 228-232.
Siemoneit U, Koeberle A, Rossi A, Dehm F, Verhoff M, Reckel S, Maier TJ, Jauch J, Northoff H, Bernhard F, Doetsch V, Sautebin L, Werz O. Inhibition of microsomal prostaglandin E2 synthase-1 as a molecular basis for the anti-inflammatory actions of boswellic acids from frankincense. Br J Pharmacol 2011, 162(1): 147-162.
Schmoe K, Rogov VV, Rogova NY, Lohr F, Guntert P, Bernhard F, Dotsch V. Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsB. Structure 2011, 19(4): 577-587.
Rozenknop A, Rogov VV, Rogova NY, Lohr F, Guntert P, Dikic I, Dotsch V. Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1. J Mol Biol 2011, 410(3): 477-487.
Rogov VV, Rogova NY, Bernhard F, Lohr F, Dotsch V. A disulfide bridge network within the soluble periplasmic domain determines structure and function of the outer membrane protein RCSF. J Biol Chem 2011, 286(21): 18775-18783.
Reckel S, Gottstein D, Stehle J, Lohr F, Verhoefen MK, Takeda M, Silvers R, Kainosho M, Glaubitz C, Wachtveitl J, Bernhard F, Schwalbe H, Guntert P, Dotsch V. Solution NMR structure of proteorhodopsin. Angew Chem Int Ed Engl 2011, 50(50): 11942-11946.
Rath P, Demange P, Saurel O, Tropis M, Daffe M, Dotsch V, Ghazi A, Bernhard F, Milon A. Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification. J Biol Chem 2011, 286(37): 32525-32532.
Matthies D, Haberstock S, Joos F, Dotsch V, Vonck J, Bernhard F, Meier T. Cell-free expression and assembly of ATP synthase. J Mol Biol 2011, 413(3): 593-603.
Ma Y, Munch D, Schneider T, Sahl HG, Bouhss A, Ghoshdastider U, Wang J, Dotsch V, Wang X, Bernhard F. Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes. J Biol Chem 2011, 286(45): 38844-38853.
Lohr F, Reckel S, Stefer S, Dotsch V, Schmidt JM. Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (alpha) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy. J Biomol NMR 2011, 50(2): 167-190.
Krstic I, Hansel R, Romainczyk O, Engels JW, Dotsch V, Prisner TF. Long-range distance measurements on nucleic acids in cells by pulsed EPR spectroscopy. Angew Chem Int Ed Engl 2011, 50(22): 5070-5074.
Kodama Y, Reese ML, Shimba N, Ono K, Kanamori E, Dotsch V, Noguchi S, Fukunishi Y, Suzuki E, Shimada I, Takahashi H. Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements. J Struct Biol 2011, 174(3): 434-442.
Keller T, Egenberger B, Gorboulev V, Bernhard F, Uzelac Z, Gorbunov D, Wirth C, Koppatz S, Dotsch V, Hunte C, Sitte HH, Koepsell H. The large extracellular loop of organic cation transporter 1 influences substrate affinity and is pivotal for oligomerization. J Biol Chem 2011, 286(43): 37874-37886.
Kantaputra PN, Malaivijitnond S, Vieira AR, Heering J, Dotsch V, Khankasikum T, Sripathomsawat W. Mutation in SAM domain of TP63 is associated with nonsyndromic cleft lip and palate and cleft palate. Am J Med Genet A 2011, 155A(6): 1432-1436.
Junge F, Haberstock S, Roos C, Stefer S, Proverbio D, Dotsch V, Bernhard F. Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins. N Biotechnol 2011, 28(3): 262-271.
Hefke F, Bagaria A, Reckel S, Ullrich SJ, Dotsch V, Glaubitz C, Guntert P. Optimization of amino acid type-specific 13C and 15N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm. J Biomol NMR 2011, 49(2): 75-84.
Hansel R, Lohr F, Foldynova-Trantirkova S, Bamberg E, Trantirek L, Dotsch V. The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions. Nucleic Acids Res 2011, 39(13): 5768-5775.
Hahn S, Achenbach J, Buscato E, Klingler FM, Schroeder M, Meirer K, Hieke M, Heering J, Barbosa-Sicard E, Loehr F, Fleming I, Doetsch V, Schubert-Zsilavecz M, Steinhilber D, Proschak E. Complementary screening techniques yielded fragments that inhibit the phosphatase activity of soluble epoxide hydrolase. ChemMedChem 2011, 6(12): 2146-2149.
Dotsch V. How to create a specific recognition for an unspecific interaction. Structure 2011, 19(5): 601-602.
Deutsch GB, Zielonka EM, Coutandin D, Weber TA, Schafer B, Hannewald J, Luh LM, Durst FG, Ibrahim M, Hoffmann J, Niesen FH, Senturk A, Kunkel H, Brutschy B, Schleiff E, Knapp S, Acker-Palmer A, Grez M, McKeon F, Dotsch V. DNA damage in oocytes induces a switch of the quality control factor TAp63alpha from dimer to tetramer. Cell 2011, 144(4): 566-576.
Deutsch GB, Zielonka EM, Coutandin D, Dotsch V. Quality control in oocytes: domain-domain interactions regulate the activity of p63. Cell Cycle 2011, 10(12): 1884-1885.
Browne G, Cipollone R, Lena AM, Serra V, Zhou H, van Bokhoven H, Dotsch V, Merico D, Mantovani R, Terrinoni A, Knight RA, Candi E, Melino G. Differential altered stability and transcriptional activity of DeltaNp63 mutants in distinct ectodermal dysplasias. J Cell Sci 2011, 124(Pt 13): 2200-2207.
Straub WE, Weber TA, Schafer B, Candi E, Durst F, Ou HD, Rajalingam K, Melino G, Dotsch V. The C-terminus of p63 contains multiple regulatory elements with different functions. Cell Death Dis 2010, 1(1): e5.
Sobhanifar S, Schneider B, Lohr F, Gottstein D, Ikeya T, Mlynarczyk K, Pulawski W, Ghoshdastider U, Kolinski M, Filipek S, Guntert P, Bernhard F, Dotsch V. Structural investigation of the C-terminal catalytic fragment of presenilin 1. Proc Natl Acad Sci U S A 2010, 107(21): 9644-9649.
Sobhanifar S, Reckel S, Junge F, Schwarz D, Kai L, Karbyshev M, Lohr F, Bernhard F, Dotsch V. Cell-free expression and stable isotope labelling strategies for membrane proteins. J Biomol NMR 2010, 46(1): 33-43.
Schwarz D, Daley D, Beckhaus T, Dotsch V, Bernhard F. Cell-free expression profiling of E. coli inner membrane proteins. Proteomics 2010, 10(9): 1762-1779.
Schneider B, Junge F, Shirokov VA, Durst F, Schwarz D, Dotsch V, Bernhard F. Membrane protein expression in cell-free systems. Methods Mol Biol 2010, 601: 165-186.
Reckel S, Sobhanifar S, Durst F, Lohr F, Shirokov VA, Dotsch V, Bernhard F. Strategies for the cell-free expression of membrane proteins. Methods Mol Biol 2010, 607: 187-212.
Novak I, Kirkin V, McEwan DG, Zhang J, Wild P, Rozenknop A, Rogov V, Lohr F, Popovic D, Occhipinti A, Reichert AS, Terzic J, Dotsch V, Ney PA, Dikic I. Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Rep 2010, 11(1): 45-51.
Ledwidge R, Hong B, Dotsch V, Miller SM. NmerA of Tn501 mercuric ion reductase: structural modulation of the pKa values of the metal binding cysteine thiols. Biochemistry 2010, 49(41): 8988-8998.
Koglin A, Doetsch V, Bernhard F. Molecular engineering aspects for the production of new and modified biosurfactants. Adv Exp Med Biol 2010, 672: 158-169.
Koeberle A, Rossi A, Zettl H, Pergola C, Dehm F, Bauer J, Greiner C, Reckel S, Hoernig C, Northoff H, Bernhard F, Dotsch V, Sautebin L, Schubert-Zsilavecz M, Werz O. The molecular pharmacology and in vivo activity of 2-(4-chloro-6-(2,3-dimethylphenylamino)pyrimidin-2-ylthio)octanoic acid (YS121), a dual inhibitor of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase. J Pharmacol Exp Ther 2010, 332(3): 840-848.
Kai L, Kaldenhoff R, Lian J, Zhu X, Dotsch V, Bernhard F, Cen P, Xu Z. Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes. PLoS One 2010, 5(9): e12972.
Junge F, Luh LM, Proverbio D, Schafer B, Abele R, Beyermann M, Dotsch V, Bernhard F. Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptor. J Struct Biol 2010, 172(1): 94-106.
Dotsch V, Bernassola F, Coutandin D, Candi E, Melino G. p63 and p73, the ancestors of p53. Cold Spring Harb Perspect Biol 2010, 2(9): a004887.
Coutandin D, Ou HD, Lohr F, Dotsch V. Tracing the protectors path from the germ line to the genome. Proc Natl Acad Sci U S A 2010, 107(35): 15318-15325.
2009 and before
Pedo M, Lohr F, D’Onofrio M, Assfalg M, Dotsch V, Molinari H. NMR studies reveal the role of biomembranes in modulating ligand binding and release by intracellular bile acid binding proteins. J Mol Biol 2009, 394(5): 852-863.
Hansel R, Foldynova-Trantirkova S, Lohr F, Buck J, Bongartz E, Bamberg E, Schwalbe H, Dotsch V, Trantirek L. Evaluation of parameters critical for observing nucleic acids inside living Xenopus laevis oocytes by in-cell NMR spectroscopy. J Am Chem Soc 2009, 131(43): 15761-15768.
Foldynova-Trantirkova S, Matulova J, Dotsch V, Lohr F, Cirstea I, Alexandov K, Breitling R, Lukes J, Trantirek L. A cost-effective amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. J Biomol Struct Dyn 2009, 26(6): 755-761.
Dotsch V. On track to tenure-track. EMBO Rep 2009, 10(9): 936-937.
Dikic I, Dotsch V. Ubiquitin linkages make a difference. Nat Struct Mol Biol 2009, 16(12): 1209-1210.
Coutandin D, Lohr F, Niesen FH, Ikeya T, Weber TA, Schafer B, Zielonka EM, Bullock AN, Yang A, Guntert P, Knapp S, McKeon F, Ou HD, Dotsch V. Conformational stability and activity of p73 require a second helix in the tetramerization domain. Cell Death Differ 2009, 16(12): 1582-1589.
Busche AE, Aranko AS, Talebzadeh-Farooji M, Bernhard F, Dotsch V, Iwai H. Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein. Angew Chem Int Ed Engl 2009, 48(33): 6128-6131.
Wagner S, Carpentier I, Rogov V, Kreike M, Ikeda F, Lohr F, Wu CJ, Ashwell JD, Dotsch V, Dikic I, Beyaert R. Ubiquitin binding mediates the NF-kappaB inhibitory potential of ABIN proteins. Oncogene 2008, 27(26): 3739-3745.
Schwarz D, Dotsch V, Bernhard F. Production of membrane proteins using cell-free expression systems. Proteomics 2008, 8(19): 3933-3946.
Rogov VV, Schmoe K, Lohr F, Rogova NY, Bernhard F, Dotsch V. Modulation of the Rcs-mediated signal transfer by conformational flexibility. Biochem Soc Trans 2008, 36(Pt 6): 1427-1432.
Reckel S, Sobhanifar S, Schneider B, Junge F, Schwarz D, Durst F, Lohr F, Guntert P, Bernhard F, Dotsch V. Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins. Proc Natl Acad Sci U S A 2008, 105(24): 8262-8267.
Koglin A, Lohr F, Bernhard F, Rogov VV, Frueh DP, Strieter ER, Mofid MR, Guntert P, Wagner G, Walsh CT, Marahiel MA, Dotsch V. Structural basis for the selectivity of the external thioesterase of the surfactin synthetase. Nature 2008, 454(7206): 907-911.
Keller T, Schwarz D, Bernhard F, Dotsch V, Hunte C, Gorboulev V, Koepsell H. Cell free expression and functional reconstitution of eukaryotic drug transporters. Biochemistry 2008, 47(15): 4552-4564.
Junge F, Schneider B, Reckel S, Schwarz D, Dotsch V, Bernhard F. Large-scale production of functional membrane proteins. Cell Mol Life Sci 2008, 65(11): 1729-1755.
Durst FG, Ou HD, Lohr F, Dotsch V, Straub WE. The better tag remains unseen. J Am Chem Soc 2008, 130(45): 14932-14933.
Dotsch V. Investigation of proteins in living bacteria with in-cell NMR experiments. Top Curr Chem 2008, 273: 203-214.
Schwarz D, Klammt C, Koglin A, Lohr F, Schneider B, Dotsch V, Bernhard F. Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies. Methods 2007, 41(4): 355-369.
Schwarz D, Junge F, Durst F, Frolich N, Schneider B, Reckel S, Sobhanifar S, Dotsch V, Bernhard F. Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. Nat Protoc 2007, 2(11): 2945-2957.
Rogov VV, Lohr F, Rogova N, Klammt C, Koglin A, Bernhard F, Dotsch V. NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain. J Biomol NMR 2007, 38(2): 165.
Reese ML, Dakoji S, Bredt DS, Dotsch V. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. Nat Struct Mol Biol 2007, 14(2): 155-163.
Ou HD, Lohr F, Vogel V, Mantele W, Dotsch V. Structural evolution of C-terminal domains in the p53 family. EMBO J 2007, 26(14): 3463-3473.
Lohr F, Hansel R, Rogov VV, Dotsch V. Improved pulse sequences for sequence specific assignment of aromatic proton resonances in proteins. J Biomol NMR 2007, 37(3): 205-224.
Klammt C, Srivastava A, Eifler N, Junge F, Beyermann M, Schwarz D, Michel H, Doetsch V, Bernhard F. Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation. FEBS J 2007, 274(13): 3257-3269.
Klammt C, Schwarz D, Eifler N, Engel A, Piehler J, Haase W, Hahn S, Dotsch V, Bernhard F. Cell-free production of G protein-coupled receptors for functional and structural studies. J Struct Biol 2007, 158(3): 482-493.
Klammt C, Schwarz D, Eifler N, Engel A, Piehler J, Haase W, Hahn S, Dotsch V, Bernhard F. Reprint of “Cell-free production of G protein-coupled receptors for functional and structural studies” [J. Struct. Biol. 158 (2007) 482-493]. J Struct Biol 2007, 159(2): 194-205.
Klammt C, Schwarz D, Dotsch V, Bernhard F. Cell-free production of integral membrane proteins on a preparative scale. Methods Mol Biol 2007, 375: 57-78.
Ikeda F, Hecker CM, Rozenknop A, Nordmeier RD, Rogov V, Hofmann K, Akira S, Dotsch V, Dikic I. Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genes. EMBO J 2007, 26(14): 3451-3462.
Hoeller D, Hecker CM, Wagner S, Rogov V, Dotsch V, Dikic I. E3-independent monoubiquitination of ubiquitin-binding proteins. Mol Cell 2007, 26(6): 891-898.
Serber Z, Selenko P, Hansel R, Reckel S, Lohr F, Ferrell JE, Jr., Wagner G, Dotsch V. Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy. Nat Protoc 2006, 1(6): 2701-2709.
Rogov VV, Rogova NY, Bernhard F, Koglin A, Lohr F, Dotsch V. A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains. J Mol Biol 2006, 364(1): 68-79.
Petrosky KY, Lohr F, Dotsch V. NMR assignment of the L27 heterodimer from LIN-2 and LIN-7 scaffold proteins. J Biomol NMR 2006, 36 Suppl 1: 15.
Nomura AM, Marnett AB, Shimba N, Dotsch V, Craik CS. One functional switch mediates reversible and irreversible inactivation of a herpesvirus protease. Biochemistry 2006, 45(11): 3572-3579.
Koglin A, Mofid MR, Lohr F, Schafer B, Rogov VV, Blum MM, Mittag T, Marahiel MA, Bernhard F, Dotsch V. Conformational switches modulate protein interactions in peptide antibiotic synthetases. Science 2006, 312(5771): 273-276.
Koglin A, Klammt C, Trbovic N, Schwarz D, Schneider B, Schafer B, Lohr F, Bernhard F, Dotsch V. Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins. Magn Reson Chem 2006, 44 Spec No: S17-23.
Klammt C, Schwarz D, Lohr F, Schneider B, Dotsch V, Bernhard F. Cell-free expression as an emerging technique for the large scale production of integral membrane protein. FEBS J 2006, 273(18): 4141-4153.
Kelly AE, Kranitz H, Dotsch V, Mullins RD. Actin binding to the central domain of WASP/Scar proteins plays a critical role in the activation of the Arp2/3 complex. J Biol Chem 2006, 281(15): 10589-10597.
Ab E, Atkinson AR, Banci L, Bertini I, Ciofi-Baffoni S, Brunner K, Diercks T, Dotsch V, Engelke F, Folkers GE, Griesinger C, Gronwald W, Gunther U, Habeck M, de Jong RN, Kalbitzer HR, Kieffer B, Leeflang BR, Loss S, Luchinat C, Marquardsen T, Moskau D, Neidig KP, Nilges M, Piccioli M, Pierattelli R, Rieping W, Schippmann T, Schwalbe H, Trave G, Trenner J, Wohnert J, Zweckstetter M, Kaptein R. NMR in the SPINE Structural Proteomics project. Acta Crystallogr D Biol Crystallogr 2006, 62(Pt 10): 1150-1161.
Trbovic N, Klammt C, Koglin A, Lohr F, Bernhard F, Dotsch V. Efficient strategy for the rapid backbone assignment of membrane proteins. J Am Chem Soc 2005, 127(39): 13504-13505.
Serber Z, Corsini L, Durst F, Dotsch V. In-cell NMR spectroscopy. Methods Enzymol 2005, 394: 17-41.
Reckel S, Lohr F, Dotsch V. In-cell NMR spectroscopy. Chembiochem 2005, 6(9): 1601-1606.
Petrosky KY, Ou HD, Lohr F, Dotsch V, Lim WA. A general model for preferential hetero-oligomerization of LIN-2/7 domains: mechanism underlying directed assembly of supramolecular signaling complexes. J Biol Chem 2005, 280(46): 38528-38536.
Nomura AM, Marnett AB, Shimba N, Dotsch V, Craik CS. Induced structure of a helical switch as a mechanism to regulate enzymatic activity. Nat Struct Mol Biol 2005, 12(11): 1019-1020.
Lohr F, Rogov VV, Shi M, Bernhard F, Dotsch V. Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues. J Biomol NMR 2005, 32(4): 309-328.
Klammt C, Schwarz D, Fendler K, Haase W, Dotsch V, Bernhard F. Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J 2005, 272(23): 6024-6038.
He C, Hus JC, Sun LJ, Zhou P, Norman DP, Dotsch V, Wei H, Gross JD, Lane WS, Wagner G, Verdine GL. A methylation-dependent electrostatic switch controls DNA repair and transcriptional activation by E. coli ada. Mol Cell 2005, 20(1): 117-129.
Rogov VV, Lohr F, Bernhard F, Dotsch V. Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain. J Biomol NMR 2004, 30(1): 103-104.
Shimba N, Kovacs H, Stern AS, Nomura AM, Shimada I, Hoch JC, Craik CS, Dotsch V. Optimization of 13C direct detection NMR methods. J Biomol NMR 2004, 30(2): 175-179.
Serber Z, Straub W, Corsini L, Nomura AM, Shimba N, Craik CS, Ortiz de Montellano P, Dotsch V. Methyl groups as probes for proteins and complexes in in-cell NMR experiments. J Am Chem Soc 2004, 126(22): 7119-7125.
Rogov VV, Bernhard F, Lohr F, Dotsch V. Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains. J Mol Biol 2004, 343(4): 1035-1048.
Klammt C, Lohr F, Schafer B, Haase W, Dotsch V, Ruterjans H, Glaubitz C, Bernhard F. High level cell-free expression and specific labeling of integral membrane proteins. Eur J Biochem 2004, 271(3): 568-580.
Shimba N, Stern AS, Craik CS, Hoch JC, Dotsch V. Elimination of 13Calpha splitting in protein NMR spectra by deconvolution with maximum entropy reconstruction. J Am Chem Soc 2003, 125(9): 2382-2383.
Shimba N, Serber Z, Ledwidge R, Miller SM, Craik CS, Dotsch V. Quantitative identification of the protonation state of histidines in vitro and in vivo. Biochemistry 2003, 42(30): 9227-9234.
Reese ML, Dotsch V. Fast mapping of protein-protein interfaces by NMR spectroscopy. J Am Chem Soc 2003, 125(47): 14250-14251.
Serber Z, Lai HC, Yang A, Ou HD, Sigal MS, Kelly AE, Darimont BD, Duijf PH, Van Bokhoven H, McKeon F, Dotsch V. A C-terminal inhibitory domain controls the activity of p63 by an intramolecular mechanism. Mol Cell Biol 2002, 22(24): 8601-8611.
Kelly AE, Ou HD, Withers R, Dotsch V. Low-conductivity buffers for high-sensitivity NMR measurements. J Am Chem Soc 2002, 124(40): 12013-12019.
Duijf PH, Vanmolkot KR, Propping P, Friedl W, Krieger E, McKeon F, Dotsch V, Brunner HG, van Bokhoven H. Gain-of-function mutation in ADULT syndrome reveals the presence of a second transactivation domain in p63. Hum Mol Genet 2002, 11(7): 799-804.
Serber Z, Richter C, Dotsch V. Carbon-detected NMR experiments to investigate structure and dynamics of biological macromolecules. Chembiochem 2001, 2(4): 247-251.
Serber Z, Ledwidge R, Miller SM, Dotsch V. Evaluation of parameters critical to observing proteins inside living Escherichia coli by in-cell NMR spectroscopy. J Am Chem Soc 2001, 123(37): 8895-8901.
Serber Z, Keatinge-Clay AT, Ledwidge R, Kelly AE, Miller SM, Dotsch V. High-resolution macromolecular NMR spectroscopy inside living cells. J Am Chem Soc 2001, 123(10): 2446-2447.
Serber Z, Dotsch V. In-cell NMR spectroscopy. Biochemistry 2001, 40(48): 14317-14323.
Ou HD, Lai HC, Serber Z, Dotsch V. Efficient identification of amino acid types for fast protein backbone assignments. J Biomol NMR 2001, 21(3): 269-273.
McGrath JA, Duijf PH, Doetsch V, Irvine AD, de Waal R, Vanmolkot KR, Wessagowit V, Kelly A, Atherton DJ, Griffiths WA, Orlow SJ, van Haeringen A, Ausems MG, Yang A, McKeon F, Bamshad MA, Brunner HG, Hamel BC, van Bokhoven H. Hay-Wells syndrome is caused by heterozygous missense mutations in the SAM domain of p63. Hum Mol Genet 2001, 10(3): 221-229.
Lin Y, Dotsch V, Wintner T, Peariso K, Myers LC, Penner-Hahn JE, Verdine GL, Wagner G. Structural basis for the functional switch of the E. coli Ada protein. Biochemistry 2001, 40(14): 4261-4271.
Gerber BO, Meng EC, Dotsch V, Baranski TJ, Bourne HR. An activation switch in the ligand binding pocket of the C5a receptor. J Biol Chem 2001, 276(5): 3394-3400.
Dotsch V. Protein-DNA interactions. Methods Enzymol 2001, 339: 343-357.
Sun ZY, Dotsch V, Kim M, Li J, Reinherz EL, Wagner G. Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies. EMBO J 1999, 18(11): 2941-2949.
Freund C, Dotsch V, Nishizawa K, Reinherz EL, Wagner G. The GYF domain is a novel structural fold that is involved in lymphoid signaling through proline-rich sequences. Nat Struct Biol 1999, 6(7): 656-660.
Zhu J, Shibasaki F, Price R, Guillemot JC, Yano T, Dotsch V, Wagner G, Ferrara P, McKeon F. Intramolecular masking of nuclear import signal on NF-AT4 by casein kinase I and MEKK1. Cell 1998, 93(5): 851-861.
Zhou P, Sun LJ, Dotsch V, Wagner G, Verdine GL. Solution structure of the core NFATC1/DNA complex. Cell 1998, 92(5): 687-696.
Yang A, Kaghad M, Wang Y, Gillett E, Fleming MD, Dotsch V, Andrews NC, Caput D, McKeon F. p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Mol Cell 1998, 2(3): 305-316.
Dotsch V, Wagner G. New approaches to structure determination by NMR spectroscopy. Curr Opin Struct Biol 1998, 8(5): 619-623.
Wolfe SA, Zhou P, Dotsch V, Chen L, You A, Ho SN, Crabtree GR, Wagner G, Verdine GL. Unusual Rel-like architecture in the DNA-binding domain of the transcription factor NFATc. Nature 1997, 385(6612): 172-176.
Feltham JL, Dotsch V, Raza S, Manor D, Cerione RA, Sutcliffe MJ, Wagner G, Oswald RE. Definition of the switch surface in the solution structure of Cdc42Hs. Biochemistry 1997, 36(29): 8755-8766.
Schiffer CA, Dotsch V. The role of protein-solvent interactions in protein unfolding. Curr Opin Biotechnol 1996, 7(4): 428-432.
Dotsch V, Wagner G. Editing for amino-acid type in CBCACONH experiments based on the 13C beta-13C gamma coupling. J Magn Reson B 1996, 111(3): 310-313.
Dotsch V, Oswald RE, Wagner G. Amino-acid-type-selective triple-resonance experiments. J Magn Reson B 1996, 110(1): 107-111.
Dotsch V, Oswald RE, Wagner G. Selective identification of threonine, valine, and isoleucine sequential connectivities with a TVI-CBCACONH experiment. J Magn Reson B 1996, 110(3): 304-308.
Dotsch V, Matsuo H, Wagner G. Amino-acid-type identification for deuterated proteins with a beta-carbon-edited HNCOCACB experiment. J Magn Reson B 1996, 112(1): 95-100.
Schiffer CA, Dotsch V, Wuthrich K, van Gunsteren WF. Exploring the role of the solvent in the denaturation of a protein: a molecular dynamics study of the DNA binding domain of the 434 repressor. Biochemistry 1995, 34(46): 15057-15067.
Dotsch V, Wider G, Siegal G, Wuthrich K. Interaction of urea with an unfolded protein. The DNA-binding domain of the 434-repressor. FEBS Lett 1995, 366(1): 6-10.
Dotsch V, Wider G, Siegal G, Wuthrich K. Salt-stabilized globular protein structure in 7 M aqueous urea solution. FEBS Lett 1995, 372(2-3): 288-290.
Dotsch V, Oswald RE, Wagner G. Water suppression by coherence selection with absorptive lineshape without loss in sensitivity. J Magn Reson B 1995, 108(3): 285-288.
